Improved immune-suppression techniques for the exongrafting of human tumours.
Improved immune-suppression techniques for the exongrafting of human tumours.
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DOI：
10.1038/bjc.1978.30
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发表时间：
1978-02
影响因子：
8.8
通讯作者：
Rostom AY
中科院分区：
文献类型：
--
作者：
Steel GG;Courtenay VD;Rostom AY
在通过胸腺切除术和全身照射免疫抑制的小鼠中检查了异种移植的人腺癌的可移植性，并将结果与移植到无胸腺（裸）小鼠中进行了比较。使用两种替代技术来防止全身照射后的骨髓衰竭：用骨髓移植物重建动物，或在照射前2天注射阿糖胞苷（Ara-C）来保护它们。结果表明，Ara-C制备的小鼠比骨髓移植或裸鼠更容易接受移植，并且它们是唯一从植入的异种移植物发生区域转移的动物。在两种类型的免疫抑制小鼠中均出现了一定程度的免疫恢复，这在免疫抑制后5周以上是明显的，并且在雌性小鼠中比雄性小鼠更明显。结论是免疫抑制小鼠上级裸鼠的短期实验，但他们可能不太满意的长期实验。
The transplantability of a xenografted human adenocarcinoma has been examined in mice that had been immune-suppressed by thymectomy and whole-body irradiation and the results have been compared with transplantation into athymic (nude) mice. Two alternative techniques were used to prevent marrow failure following whole-body irradiation: reconstituting the animals with a marrow graft, or protecting them by an injection of cytosine arabinoside (Ara-C) 2 days before the irradiation. The results show that the Ara-C-prepared mice were more receptive to transplantation than marrow-grafted or nude mice, and they were the only animals that developed regional metastases from implanted xenografts. Some recovery of immunity occurred in both types of immune-suppressed mice, which was evident more than 5 weeks after immune-suppression and which was more marked in females than in males. It was concluded that the immune-suppressed mice were superior to nude mice for short-term experiments but they may be less satisfactory for long-term experiments.