Evaluation of mucosal bacteria and histopathology, clinical disease activity and expression of Toll-like receptors in German shepherd dogs with chronic enteropathies
Evaluation of mucosal bacteria and histopathology, clinical disease activity and expression of Toll-like receptors in German shepherd dogs with chronic enteropathies
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DOI：
10.1016/j.vetmic.2010.05.025
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发表时间：
2010-12-15
影响因子：
3.3
通讯作者：
Suchodolski, J. S.
中科院分区：
文献类型：
--
作者：
Allenspach, K.;House, A.;Suchodolski, J. S.
犬慢性肠病的发病机制可能涉及肠道免疫系统和肠腔细菌之间的相互作用。德国牧羊犬（GSD）特别容易患慢性肠病。为探讨先天免疫模式识别受体（TLR）在GSD慢性肠病中的表达模式、临床疾病活动性和组织病理学严重程度，对13只GSD患者和10只健康灰狗的十二指肠、结肠和回肠进行了粘膜活检。使用已发表的评分系统客观评估犬的临床和组织学疾病严重程度。通过构建16S rRNA基因文库来评估十二指肠微生物群的多样性。实时荧光定量PCR检测GSD犬十二指肠、回肠和结肠组织中TLR2、TLR4、TLR5和TLR9的表达，结果显示GSD犬各肠段TLR4的表达均升高，而TLR5的表达较健康犬低。GSD的十二指肠微生物群与灰狗的十二指肠微生物群显著不同，属于芽孢杆菌和丹毒丝菌纲以及乳杆菌目、放线菌目和丹毒丝菌目的165个rRNA基因序列过度表达。这些发现可以指出GSD慢性肠病的独特发病机制，分别具有TLR4和TLR5的差异高表达和低表达，以及可能起作用的乳杆菌目的特定成员的比例增加。(C)2010 Elsevier BV保留所有权利。
The pathogenesis of chronic enteropathies in dogs likely involves an interaction between the intestinal immune system and luminal intestinal bacteria. German shepherd dogs (GSD) are particularly predisposed to chronic enteropathies. The present study sought to evaluate expression patterns of certain pattern recognition receptors of the innate immunity (Toll-like receptors, TLR), clinical disease activity and histopathological severity in GSD with chronic enteropathies.Mucosal biopsies were collected from the duodenum, colon and ileum of 13 affected GSD and 10 healthy greyhounds as controls. Dogs were objectively assessed using published scoring systems for clinical and histological severity of disease. Diversity of the duodenal microbiota was assessed by construction of 16S rRNA gene libraries. Expression of TLR2, TLR4, TLR5 and TLR9 in biopsies of the duodenum, ileum and colon was assessed by quantitative real-time PCR.TLR4 expression was increased in all intestinal segments in GSD, however, TLR5 expression was very low compared to the healthy dogs. The microbiota in the duodenum of GSDs was significantly different to that of the greyhounds, with an over-representation of 165 rRNA gene sequences belonging to the classes of Bacilli, and Erysipelotrichi, and to the orders of Lactobacillales, Actinomycetales and Erysipelotrichales.These findings could point to a distinct pathogenesis of chronic enteropathies in GSD, with differentially high and low expression of TLR4 and TLR5, respectively, and increased proportions of specific members of the Lactobacillales potentially playing a role. (C) 2010 Elsevier B.V. All rights reserved.