PASSIVE PROTECTION AGAINST ROTAVIRUS-INDUCED DIARRHEA BY MONOCLONAL-ANTIBODIES TO THE HETEROTYPIC NEUTRALIZATION DOMAIN OF VP7 AND THE VP8 FRAGMENT OF VP4
PASSIVE PROTECTION AGAINST ROTAVIRUS-INDUCED DIARRHEA BY MONOCLONAL-ANTIBODIES TO THE HETEROTYPIC NEUTRALIZATION DOMAIN OF VP7 AND THE VP8 FRAGMENT OF VP4
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DOI：
10.1128/jcm.27.4.780-782.1989
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发表时间：
1989-04-01
影响因子：
9.4
通讯作者：
GREENBERG, HB
中科院分区：
文献类型：
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作者：
MATSUI, SM;OFFIT, PA;GREENBERG, HB
使用鼠模型来确定针对 VP7 (MAb 57-8) 或 VP4 的 VP8 片段 (MAb M14) 的具有异型特异性的中和单克隆抗体 (MAb) 是否被动保护小鼠免受各种轮状病毒株的攻击。 （基因 4 产物，一种外衣壳蛋白，传统上称为 VP3。然而，有人建议将轮状病毒基因 4 产物命名为 VP4。基因 3 产物，一种核心蛋白，最近已被鉴定并命名为 VP3 [M. Liu, P. A. Offit, and M. K. Estes, Virology 163-28-32, 1988]。）当用强毒血清型 3、4 或 6 轮状病毒攻击时，MAb 57-8 不会出现腹泻，而接种 MAb M14 的那些则被动保护免受血清型 3 或 6 轮状病毒的攻击，正如体外中和试验所预测的那样。然而，当小鼠受到其他血清型轮状病毒攻击时，这些单克隆抗体并不能保护小鼠免受感染。我们得出的结论是，每种表面蛋白上的特定中和表位能够介导针对一种或多种轮状病毒血清型的保护。
A murine model was used to determine whether neutralizing monoclonal antibodies (MAbs) with heterotypic specificity directed to VP7 (MAb 57-8) or to the VP8 fragment of VP4 (MAb M14) passively protect mice against challenge with various strains of rotavirus. (The gene 4 product, an outer capsid protein, has traditionally been called VP3. It has been proposed, however, that the rotavirus gene 4 product be named VP4. The gene 3 product, a core protein, has been identified recently and named VP3 [M. Liu, P. A. Offit, and M. K. Estes, Virology 163-28-32, 1988].) Suckling mice orally inoculated with MAb 57-8 did not develop diarrhea when challenged with virulent serotype 3, 4, or 6 rotaviruses, while those inoculated with MAb M14 were passively protected from challenge with serotype 3 or 6 rotaviruses, as predicted by in vitro neutralization tests. These MAbs, however, did not protect mice from infection when the mice were challenged with rotaviruses of other serotypes. We conclude that specific neutralization epitopes on each surface protein are capable of mediating protection against one or several rotavirus serotypes.