Connective tissue growth factor mRNA expression pattern in cartilages is associated with their type I collagen expression
Connective tissue growth factor mRNA expression pattern in cartilages is associated with their type I collagen expression
复制标题
DOI：
10.1016/j.bone.2003.07.010
复制
发表时间：
2003-12-01
期刊：
影响因子：
4.1
通讯作者：
Takano-Yamamoto, T
中科院分区：
文献类型：
--
作者：
Fukunaga, T;Yamashiro, T;Takano-Yamamoto, T
结缔组织生长因子(CTGF)是一种由即刻早期基因编码的分泌性蛋白，是CCN家族的一员。在体外，CTGF直接调节软骨细胞的增殖和分化；然而，先前的研究表明，CTGF仅定位于E18小鼠胚胎肋软骨中肥大的软骨细胞。应用原位杂交和免疫组织化学方法，研究了CTGF在不同类型软骨中的表达，包括股骨生长板软骨、肋骨软骨、股骨关节软骨、下颌骨髁状突软骨和下颌升支骨折愈合过程中形成的软骨。为了确定表达CTGF的细胞的特征，我们还分析了I型、II型和X型胶原mRNA表达的分布。在这些不同类型的软骨中，我们发现了CTGF mRNA和蛋白表达的不同模式。在生长板软骨和肋软骨中，CTGF mRNA和蛋白在表达X型胶原的肥大软骨细胞中有表达，而在表达II型胶原的增殖期软骨细胞中表达较少，在髁突软骨、关节软骨和骨折愈合过程中出现的软骨中也有表达。而生长板软骨和肋软骨的I型胶原表达均为阴性，在增殖的软骨细胞中CTGF mRNA表达稀少。我们首次发现CTGF mRNA可以在5种不同类型的软骨中差异表达，与表达I型胶原的软骨相关。此外，CTGF mRNA在含有I型胶原mRNA的软骨中的空间分布提示其在软骨的早期分化、增殖和终末分化中起作用。(C)2003 Elsevier Inc.保留所有权利。
Connective tissue growth factor (CTGF) has been identified as a secretory protein encoded by an immediate early gene and is a member of the CCN family. In vitro CTGF directly regulates the proliferation and differentiation of chondrocytes; however, a previous study showed that it was localized only in the hypertrophic chondrocytes in the costal cartilages of E 18 mouse embryos. We described the expression of CTGF mRNA and protein in chondrocytes of different types of cartilages, including femoral growth plate cartilage, costal cartilage, femoral articular cartilage, mandibular condylar cartilage, and cartilage formed during the healing of mandibular ramus fractures revealed by in situ hybridization and immunohistochemistry. To characterize the CTGF-expressing cells, we also analyzed the distribution of the type I, type II, and type X collagen mRNA expression. Among these different types of cartilages we found distinct patterns of CTGF mRNA and protein expression. Growth plate cartilage and the costal cartilage showed localization of CTGF mRNA and protein in the hypertrophic chondrocytes that expressed type X collagen mRNA with less expression in proliferating chondrocytes that expressed type II collagen mRNA, whereas it was also expressed in the proliferating chondrocytes that expressed type I collagen mRNA in the condylar cartilage, the articular cartilage, and the cartilage appearing during fracture healing. In contrast, the growth plate cartilages or the costal cartilages were negative for type I collagen and showed sparse expression of CTGF mRNA in the proliferating chondrocytes. We found for the first time that CTGF mRNA could be differentially expressed in five different types of cartilage associated with those expressing type I collagen. Moreover, the spatial distribution of CTGF mRNA in the cartilages with type I collagen mRNA suggested its roles in the early differentiation, as well as in the proliferation and the terminal differentiation, of those cartilages. (C) 2003 Elsevier Inc. All rights reserved.