Immunochemical approaches to gene probe assays.
Immunochemical approaches to gene probe assays.
复制标题
基因探针测定的免疫化学方法。
DOI：
10.1016/0003-2697(87)90467-2
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发表时间：
1987
影响因子：
2.9
通讯作者：
Rashtchian,A
中科院分区：
文献类型：
--
作者：
Stollar,BD;Rashtchian,A
螺旋核酸特异性抗体可用于杂交DNA和/或RNA的测定。检测可以使用放射性或非放射性检测系统。RNA-DNA杂交体特异性抗体适用于测定固定在塑料或硝化纤维素上的杂交螺旋，无论螺旋是在溶液中形成还是在固相载体上形成。另外，抗rna - dna杂交抗体可以固定并用于捕获溶液中形成的杂交体，从而产生具有高信噪比的测定。该方法已应用于生物样品中空肠弯曲杆菌核糖体RNA的检测。
Antibodies specific for helical nucleic acids can be applied to assays for hybridized DNA and/or RNA. The assays can use either radioactive or nonradioactive detection systems. Antibodies specific for RNA-DNA hybrids are applicable to assays for measuring hybrid helices that are immobilized on plastic or nitrocellulose, whether the helices are preformed in solution or are formed on the solid-phase support. Alternatively, anti-RNA-DNA hybrid antibodies can be immobilized and used to capture hybrids formed in solution, resulting in an assay with a high signal-to-noise ratio. It has been applied to a test for the presence of ribosomal RNA of Campylobacter jejuni in biological samples.