Identification of novel lumbricin homologues in Eisenia andrei earthworms
Identification of novel lumbricin homologues in Eisenia andrei earthworms
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DOI：
10.1016/j.dci.2018.09.001
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发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Engelmann, Peter
中科院分区：
文献类型：
--
作者：
Bodo, Kornelia;Boros, Akos;Engelmann, Peter
Lumbricin 及其同源抗菌肽通常从环节动物中分离出来。在这份报告中，在 Eisenia andrei 蚯蚓中鉴定出了蚓素的 mRNA 以及偶然发现的一种新的蚓素相关 mRNA 序列。确定的E. andrei 蚓素和蚓素相关肽的mRNA序列由477和575个核苷酸组成。富含脯氨酸的 E. andrei lumbricin 和相关肽的前体分别含有 63 和 59 个氨基酸。系统发育分析表明与其他环节动物蚓素有密切关系。两种 mRNA 的最高表达出现在肠道近端部分（咽、砂囊），而其他测试器官则具有中等水平（体壁、中肠、卵巢、后肾、精囊、腹神经索）或低水平（体腔细胞）。在个体发生过程中，它们的表达揭示了胚胎的持续增加。体内革兰氏阳性菌攻击 48 小时后，体腔细胞中的两种 mRNA 均显着升高，而革兰氏阴性菌或酵母聚糖刺激则没有可检测到的影响。
Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.