IMMUNOCYTOCHEMICAL AND ELECTRON-MICROSCOPIC CHARACTERIZATION OF MACROPHAGE MICROGLIA CELLS AND EXPRESSION OF CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX IN THE PINEAL-GLAND OF THE RAT
IMMUNOCYTOCHEMICAL AND ELECTRON-MICROSCOPIC CHARACTERIZATION OF MACROPHAGE MICROGLIA CELLS AND EXPRESSION OF CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX IN THE PINEAL-GLAND OF THE RAT
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DOI：
10.1007/bf00302731
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发表时间：
1993-05-01
影响因子：
3.6
通讯作者：
MCNULTY, JA
中科院分区：
文献类型：
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作者：
PEDERSEN, EB;FOX, LM;MCNULTY, JA
利用巨噬细胞/小胶质细胞单克隆抗体MRC OX-42和ED1，以及识别主要组织相容性复合体（MHC） II类抗原的MRC OX-6，对大鼠松果体间质细胞进行免疫细胞化学表征。用抗GFAP的多克隆抗体鉴定星形胶质细胞。OX-42和/或ED1免疫阳性的细胞遍布腺体；它们主要沿着血管周围间隙延伸，偶尔在腺体实质内延伸。在超微结构上，这些ox -42阳性细胞的特征是细胞核具有稀疏的异染色质和细胞质空泡/溶酶体。表达MHCⅱ类抗原的细胞分布和形态与ox -42免疫阳性细胞相似，提示松果体巨噬细胞/小胶质细胞发挥抗原提呈细胞的作用。gfap阳性星形胶质细胞集中在松果体柄进入腺体的近端。抗原提呈细胞在脑室周围神经内分泌腺的出现具有重要的功能意义，因为这些细胞可能是神经免疫调节机制的介质，并参与某些疾病状态，如自身免疫性松果体炎。
Interstitial cells in the pineal gland of the rat were characterized immunocytochemically using the monoclonal antibodies MRC OX-42 and ED1 for macrophages/microglia, and MRC OX-6, which recognizes major histocompatibility complex (MHC) class II antigen. A polyclonal antibody against GFAP was used to identify astrocytes. Cells immunopositive for OX-42 and/or ED1 were distributed throughout the gland; they extended processes primarily along the perivascular spaces and occasionally within the parenchyma of the gland. Ultrastructurally, these OX-42-positive cells were characterized by a nucleus with sparse heterochromatin and cytoplasmic vacuoles/lysosomes. Cells expressing MHC class II antigen had a distribution and morphology similar to OX-42-immunopositive cells, suggesting that pineal macrophages/microglia play a role as antigen-presenting cells. GFAP-positive astrocytes were concentrated at the proximal end of the pineal where the pineal stalk enters the gland. The occurrence of antigen-presenting cells in this circumventricular neuroendocrine gland has important functional implications as these cells may be mediators of neuroimmunomodulatory mechanisms, and involved in certain disease states such as autoimmune pinealitis.