Type II glucocorticoid receptors in the CNS regulate metabolism in ob/ob mice independent of protein synthesis.
Type II glucocorticoid receptors in the CNS regulate metabolism in ob/ob mice independent of protein synthesis.
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CNS 中的 II 型糖皮质激素受体调节 ob/ob 小鼠的代谢，不依赖于蛋白质合成。
DOI：
10.1152/ajpendo.1994.266.3.e427
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Romsos,DR
中科院分区：
文献类型：
--
作者：
Chen,HL;Romsos,DR
在肾上腺切除的ob/ob小鼠中，通过GDP结合评估，单次脑室内注射地塞米松迅速（30分钟内）使棕色脂肪组织产热减少25%，并使血浆胰岛素增加两倍。本研究调查了介导这些作用的皮质激素受体的类型，并确定蛋白质合成是否是ob/ob小鼠表达这些糖皮质激素作用所必需的。侧脑室注射醛固酮（一种I型皮质激素受体激动剂）在改变肾上腺切除ob/ob小鼠外周代谢方面无效，而RU-486（一种II型皮质激素受体拮抗剂）则消除了地塞米松的作用。因此，II型皮质激素样受体，而不是I型受体，介导地塞米松在肾上腺切除ob/ob小鼠中的快速作用。茴香霉素（0.5 mg）皮下给药几乎完全抑制（~ 92%）脑蛋白质合成，但茴香霉素没有消除肾上腺切除ob/ob小鼠中地塞米松的快速作用。因此，蛋白质合成不是地塞米松在肾上腺切除的ob/ob小鼠中快速作用的先决条件。
A single intracerebroventricular injection of dexamethasone rapidly (within 30 min) decreases brown adipose tissue thermogenesis by 25% as assessed by GDP binding and increases plasma insulin twofold in adrenalectomized ob/ob mice. The present study investigated the type of corticoid receptor(s) that mediate these effects and determined whether protein synthesis was necessary for expression of these glucocorticoid actions in ob/ob mice. Intracerebroventricular injection of aldosterone (a type I-corticoid receptor agonist) was ineffective in altering peripheral metabolism in adrenalectomized ob/ob mice, whereas RU-486 (a type II-corticoid receptor antagonist) abolished the effects of dexamethasone. Thus type II-like corticoid receptors, not type I receptors, mediated the rapid effects of dexamethasone in adrenalectomized ob/ob mice. Anisomycin (0.5 mg) administered subcutaneously almost completely suppressed (-92%) cerebral protein synthesis, but anisomycin did not abolish the rapid effects of dexamethasone in adrenalectomized ob/ob mice. Thus protein synthesis is not a prerequisite for rapid effects of dexamethasone in adrenalectomized ob/ob mice.