Transcriptomic response of durum wheat to nitrogen starvation.
Transcriptomic response of durum wheat to nitrogen starvation.
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DOI：
10.1038/s41598-017-01377-0
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发表时间：
2017-04-26
影响因子：
4.6
通讯作者：
Sonnante G
中科院分区：
文献类型：
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作者：
Curci PL;Aiese Cigliano R;Zuluaga DL;Janni M;Sanseverino W;Sonnante G
氮素是小麦生长发育的重要限制因子，影响小麦的产量。本研究采用转录组学方法研究了硬粒小麦根、叶/茎、旗叶和穗对灌浆期N长期饥饿的反应。斯韦沃氮素胁迫对株高、分蘖、旗叶面积、穗粒性状和全氮含量产生负效应。RNA-seq数据显示了4，626个差异表达基因（DEG）。在根中观察到最多的转录组变化，有3，270个DEG，而在叶/茎中发现963个，旗叶中发现470个，穗组织中发现355个。共鉴定了799个基因本体（GO）术语，分别在上调和下调的基因中有180个和619个。在最受关注的GO类别中，氮化合物代谢、碳代谢和光合作用是最具代表性的。重点介绍了氮素转运蛋白、氮素同化相关基因、沿着转录因子、蛋白激酶和其他与胁迫相关的基因。这些结果提供了有关硬粒小麦对慢性氮胁迫的转录组学反应的有价值的信息，这可能有助于今后提高氮素利用效率。
Nitrogen (N) is a key macronutrient representing a limiting factor for plant growth and development and affects productivity in wheat. In this study, durum wheat response to N chronic starvation during grain filling was investigated through a transcriptomic approach in roots, leaves/stems, flag leaf and spikes of cv. Svevo. Nitrogen stress negatively influenced plant height, tillering, flag leaf area, spike and seed traits, and total N content. RNA-seq data revealed 4,626 differentially expressed genes (DEGs). Most transcriptomic changes were observed in roots, with 3,270 DEGs, while 963 were found in leaves/stems, 470 in flag leaf, and 355 in spike tissues. A total of 799 gene ontology (GO) terms were identified, 180 and 619 among the upregulated and downregulated genes, respectively. Among the most addressed GO categories, N compound metabolism, carbon metabolism, and photosynthesis were mostly represented. Interesting DEGs, such as N transporters, genes involved in N assimilation, along with transcription factors, protein kinases and other genes related to stress were highlighted. These results provide valuable information about the transcriptomic response to chronic N stress in durum wheat, which could be useful for future improvement of N use efficiency.