Interaction of murine granulocyte-macrophage progenitors and supporting stroma involves a recognition mechanism with galactosyl and mannosyl specificities.
Interaction of murine granulocyte-macrophage progenitors and supporting stroma involves a recognition mechanism with galactosyl and mannosyl specificities.
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小鼠粒细胞-巨噬细胞祖细胞和支持基质的相互作用涉及具有半乳糖基和甘露糖基特异性的识别机制。
DOI：
10.1172/jci113260
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Tavassoli,M
中科院分区：
文献类型：
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作者：
Aizawa,S;Tavassoli,M
为了研究粒细胞-巨噬细胞祖细胞（CFU-C）“归巢”的分子基础，我们通过糖与牛血清白蛋白的共价连接合成了探针。在存在和不存在这些探针的情况下建立长期骨髓培养。在半乳糖和甘露糖基探针存在下，上清液和贴壁层中总细胞和CFU-C的产生停止，鹅卵石（代表与基质结合的CFU-C）消失。Focusyl探针和扩散糖对这些参数没有影响。这些研究表明，对半乳糖和甘露糖基残基具有特异性的膜凝集素可能是CFU-C与支持基质结合的原因。为了确定CFU-C是否具有这些特异性的归巢受体，我们用这些新糖蛋白探针在骨髓细胞悬浮液中诱导凝集。仅用半乳糖和甘露糖醇探针观察到选择性凝集。结果表明，CFU-C归巢受体是对半乳糖基和甘露糖基残基具有特异性的膜凝集素。图片
To study the molecular basis of "homing" of granulocyte-macrophage progenitors (CFU-C), we synthesized probes by covalent linking of sugars to bovine serum albumin. Long-term marrow cultures were established in the presence and absence of these probes. In the presence of galactosyl and mannosyl probes, total cell and CFU-C production in the supernate as well as the adherent layer were halted, and cobblestones (representing CFU-C bound to stroma) disappeared. Fucosyl probe and diffusible sugars had no effect on these parameters. These studies suggested membrane lectins with specificity for galactosyl and mannosyl residues may be responsible for the binding of CFU-C to supporting stroma. To determine if CFU-C possesses homing receptors with these specificities, we induced agglutination in marrow cell suspensions with these neoglycoprotein probes. Selective agglutination was observed only by galactosyl and mannosyl probes. The results suggest that CFU-C homing receptors are membrane lectins with specificity for galactosyl and mannosyl residues.Images