Towards in vivo nuclear microscopy: iodine-125 imaging in mice using micro-pinholes
Towards in vivo nuclear microscopy: iodine-125 imaging in mice using micro-pinholes
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DOI：
10.1007/s00259-002-0805-6
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发表时间：
2002-07-01
影响因子：
9.1
通讯作者：
Cherry, SR
中科院分区：
文献类型：
--
作者：
Beekman, FJ;McElroy, DP;Cherry, SR
配备准直器的位置灵敏伽马辐射探测器几十年来一直用于活体成像实验动物和人类体内放射性标记分子的分布。到目前为止，啮齿类动物获得的最佳图像分辨率约为1毫米。在这里，我们展示了如何构建一个基本和紧凑的伽马相机，用于在小动物体内进行放射性核素成像，空间分辨率要高得多。通过将致密、成形的微针孔与碘-125相结合，分辨率得到了提高。碘-125是一种低能量排放的同位素，易于并入各种分子，并通过其他适合于核医学成像的碘同位素直接转化到临床。用这台简单的台式相机，以高达200微米的分辨率获得了测试分布和小鼠甲状腺的I-125图像。讨论了超高分辨率发射层析成像可能的应用和扩展。
Position-sensitive gamma-radiation detectors equipped with collimators have been used for in vivo imaging of the distribution of radiolabelled molecules in laboratory animals and humans for several decades. To date, the best image resolution achieved in a rodent is on the order of 1 mm. Here we demonstrate how a basic and compact gamma camera can be constructed for in vivo radionuclide imaging in small animals, at much higher spatial resolution. Resolution improvements were obtained by combining dense, shaped, micro-pinhole apertures with iodine-125, an isotope with low energy emissions, ease of incorporation into a wide range of molecules, and straightforward translation into the clinic via other isotopes of iodine that are suitable for nuclear medicine imaging. I-125 images of test distributions and a mouse thyroid have been obtained at a resolution of as high as 200 mum using this simple bench-top camera. Possible future applications and extension to ultra-high-resolution emission tomography are discussed.