Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea: Copepoda)
Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea: Copepoda)
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DOI：
10.1007/s10126-002-0033-x
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发表时间：
2002-07-01
影响因子：
3
通讯作者：
Nishida, S
中科院分区：
文献类型：
--
作者：
Machida, RJ;Miya, MU;Nishida, S
完整的核苷酸序列的线粒体基因组的确定为harpacticoid桡足类，Tigriopus的（甲壳纲），使用的方法，采用长的聚合酶链反应技术和引物步移。虽然基因组（14，628 bp）包含与其他后生动物相同的37个基因（2个核糖体RNA，22个转运RNA和13个蛋白质编码基因），但以前报道的基因顺序与T.日本人此外，所有的基因都在一条链上编码，这与大多数后生动物的线粒体基因组不同。tRNA和rRNA基因的大小明显减少，导致节肢动物谱系中最小的线粒体基因组之一。虽然这种大规模的基因重排似乎发生在T。然而，没有一种机制可以粗略地解释这种基因排列的古怪性。
The complete nucleotide sequence of the mitochondrial genome was determined for a harpacticoid copepod, Tigriopus japonicus (Crustacea), using an approach that employs a long polymerase chain reaction technique and primer walking. Although the genome (14,628 bp) contained the same set of 37 genes (2 ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as found in other metazoan animals, none of the previously reported gene orders were comparable to that of T. Japonicus. Furthermore, all genes were encoded on one strand, unlike the mitochondrial genomes of most metazoan animals. Size reductions were notable for tRNA and rRNA genes, resulting in one of the smallest mitochondrial genomes in the arthropod lineage. Although it appears that such large-scale gene rearrangements have occurred in the ancestral species of T. japonicus, none of the proposed mechanisms parsimoniously account for this eccentric gene arrangement.