Maternal circulating leukocytes display early chemotactic responsiveness during late gestation.
Maternal circulating leukocytes display early chemotactic responsiveness during late gestation.
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DOI：
10.1186/1471-2393-13-s1-s8
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发表时间：
2013
影响因子：
3.1
通讯作者：
Olson DM
中科院分区：
文献类型：
--
作者：
Gomez-Lopez N;Tanaka S;Zaeem Z;Metz GA;Olson DM
分娩被广泛描述为一种免疫反应；然而，目前尚不清楚这是如何触发的。我们假设，分娩早期的一个事件是外周白细胞对生殖组织表达的趋化刺激的反应性增加，这先于组织趋化活性、子宫激活和全身孕酮/雌二醇转移的表达。在妊娠第17、20和22天（足月妊娠）采集妊娠龙-埃文斯大鼠的组织和血液。我们采用了经过验证的Boyden室试验、流式细胞术、定量实时聚合酶链反应和酶联免疫吸附试验。用GD22子宫和子宫颈提取物检测GD20母系外周白细胞时，我们发现GD20母系外周白细胞比GD17母系外周白细胞迁移更多。GD20上的白细胞也显示出趋化因子（C-C基序）配体2 （Ccl2）基因表达的显著增加，这与外周粒细胞比例的增加和B细胞和单核细胞比例的减少有关。组织趋化活性和特异性趋化因子（CCL2、趋化因子（C-X-C基序）配体1/CXCL1和CXCL10）在GD17至GD20期间基本不变，仅在GD22时升高。宫颈组织中CXCL10在GD20处达到峰值。正如预期的那样，前列腺素F2α受体和催产素受体基因的表达在GD20和22之间显著增加。GD20和gd22期间外周血清、宫颈和子宫组织提取物的孕酮浓度下降，雌二醇-17β浓度升高。母体循环白细胞表现出早期的趋化反应，这导致它们浸润到子宫，在那里它们可能参与分娩过程。
Parturition has been widely described as an immunological response; however, it is unknown how this is triggered. We hypothesized that an early event in parturition is an increased responsiveness of peripheral leukocytes to chemotactic stimuli expressed by reproductive tissues, and this precedes expression of tissue chemotactic activity, uterine activation and the systemic progesterone/estradiol shift. Tissues and blood were collected from pregnant Long-Evans rats on gestational days (GD) 17, 20 and 22 (term gestation). We employed a validated Boyden chamber assay, flow cytometry, quantitative real time-polymerase chain reaction, and enzyme-linked immunosorbent assays. We found that GD20 maternal peripheral leukocytes migrated more than those from GD17 when these were tested with GD22 uterus and cervix extracts. Leukocytes on GD20 also displayed a significant increase in chemokine (C-C motif) ligand 2 (Ccl2) gene expression and this correlated with an increase in peripheral granulocyte proportions and a decrease in B cell and monocyte proportions. Tissue chemotactic activity and specific chemokines (CCL2, chemokine (C-X-C motif) ligand 1/CXCL1, and CXCL10) were mostly unchanged from GD17 to GD20 and increased only on GD22. CXCL10 peaked on GD20 in cervical tissues. As expected, prostaglandin F2α receptor and oxytocin receptor gene expression increased dramatically between GD20 and 22. Progesterone concentrations fell and estradiol-17β concentrations increased in peripheral serum, cervical and uterine tissue extracts between GD20 and 22. Maternal circulating leukocytes display early chemotactic responsiveness, which leads to their infiltration into the uterus where they may participate in the process of parturition.