MicroRNA expression and regulation in mouse uterus during embryo implantation
MicroRNA expression and regulation in mouse uterus during embryo implantation
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小鼠子宫胚胎着床过程中MicroRNA的表达和调控
DOI：
10.1074/jbc.m800406200
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发表时间：
2008-08-22
影响因子：
4.8
通讯作者：
Yang, Zeng-Ming
中科院分区：
文献类型：
--
作者：
Hu, Shi-Jun;Ren, Gang;Yang, Zeng-Ming
MicroRNAs(MiRNAs)是存在于多种生物中的21-24个核苷酸的非编码RNA。虽然已经克隆或预测了数百个miRNAs，但只有很少的miRNAs被功能鉴定。胚胎着床是哺乳动物生殖过程中的关键步骤。在胚胎着床过程中，许多基因在小鼠子宫中发生了显著的变化。然而，小鼠子宫中着床部位和着床间部位之间的miRNA表达谱仍不清楚。本研究利用miRNA微阵列技术研究了小鼠子宫中miRNAs在着床部位和着床间部位的差异表达。Northern印迹和原位杂交均证实，与植入间相比，有8个miRNAs在植入部位表达上调。MIR-21在妊娠第5天着床部位的瘤下基质细胞中高表达。由于在假孕和延迟着床的小鼠子宫中未检测到miR-21的表达，因此在着床部位miR-21的表达受活跃的囊胚的调节。此外，我们还证明了RECK是miR-21的靶基因。我们的数据提示miR-21可能在胚胎着床过程中发挥关键作用。
MicroRNAs (miRNAs) are 21-24-nucleotide non-coding RNAs found in diverse organisms. Although hundreds of miRNAs have been cloned or predicted, only very few miRNAs have been functionally characterized. Embryo implantation is a crucial step in mammalian reproduction. Many genes have been shown to be significantly changed in mouse uterus during embryo implantation. However, miRNA expression profiles in the mouse uterus between implantation sites and inter-implantation sites are still unknown. In this study, miRNA microarray was used to examine differential expression of miRNAs in the mouse uterus between implantation sites and inter-implantation sites. Compared with inter-implantation sites, there were 8 up-regulated miRNAs at implantation sites, which were confirmed by both Northern blot and in situ hybridization. miR-21 was highly expressed in the subluminal stromal cells at implantation sites on day 5 of pregnancy. Because miR-21 was not detected in mouse uterus during pseudopregnancy and under delayed implantation, miR-21 expression at implantation sites was regulated by active blastocysts. Furthermore, we showed that Reck was the target gene of miR-21. Our data suggest that miR-21 may play a key role during embryo implantation.