Synthesis of α-glycosidase hybrid nano-flowers and their application for enriching and screening α-glycosidase inhibitors
Synthesis of α-glycosidase hybrid nano-flowers and their application for enriching and screening α-glycosidase inhibitors
复制标题
α-糖苷酶杂化纳米花的合成及其在富集和筛选α-糖苷酶抑制剂中的应用
DOI：
10.1039/c7nj03545d
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发表时间：
2018-01-07
影响因子：
3.3
通讯作者：
Yin, Xiao-Ying
中科院分区：
文献类型：
--
作者：
Qian, Kang;Wang, Han;Yin, Xiao-Ying
为了快速、准确地筛选中草药中的α-糖苷酶抑制剂化合物，我们合成了一种新型纳米亲和材料，以实现α-糖苷酶抑制剂的靶向筛选和富集。这种纳米亲和材料是通过α-糖苷酶和Cu2+自组装合成的。系统分析揭示了这种纳米材料的花状结构，直径为7.564毫米。与游离酶相比，酶活力最高为214.03%。该新材料用于筛选和富集大黄中的α-糖苷酶抑制剂。检测到十种化合物。其中，L-表儿茶素和蒽醌表现出抑制活性。这种新型纳米混合亲和材料可以快速筛选和分离复杂系统中的活性成分。
To rapidly and accurately screen compounds present in traditional Chinese herbal medicines for alpha-glycosidase inhibitors, we synthesized a novel nano-affinity material to enable the targeted screening and enrichment of alpha-glycosidase inhibitors. This nano-affinity material was synthesized by self-assembly involving alpha-glycosidase and Cu2+. A systematic analysis revealed the flower-like structure of this nano-material, with a diameter of 7.564 mm. The highest enzyme activity was 214.03% compared to free enzymes. This novel material was used to screen and enrich alpha-glycosidase inhibitors from Chinese rhubarb. Ten compounds were detected. Of these, L-epicatechin and anthraquinone displayed inhibitory activity. This novel nano-hybrid affinity material allows the rapid screening and separation of active components in complex systems.