ANTIGEN PRESENTATION BY IA+ B-CELL HYBRIDOMAS TO H-2-RESTRICTED T-CELL HYBRIDOMAS
ANTIGEN PRESENTATION BY IA+ B-CELL HYBRIDOMAS TO H-2-RESTRICTED T-CELL HYBRIDOMAS
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DOI：
10.1073/pnas.79.11.3604
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
MARRACK, P
中科院分区：
文献类型：
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作者：
KAPPLER, J;WHITE, J;MARRACK, P
将Ia+，H-2d BALB/淋巴瘤细胞系L10.A2J与其他H-2单倍型小鼠去T细胞株的脾细胞融合。其中一部分杂交瘤细胞在融合过程中表达正常脾细胞伴侣的Ia抗原，证明它们将各种抗原呈递给一组抗原特异的I区限制性T细胞杂交瘤。对三个克隆的杂交种进行了详细的研究。抗原提呈可被抗Ia单抗特异性抑制。同时表达了I-A和I-E分子，其中一例检测了H-2d和H-2b单倍型之间的杂交I-A和I-E分子。这些Ia+B细胞杂交瘤为研究I区编码分子在抗原呈递给T细胞中的作用提供了一套有用的工具。
The Ia+, H-2d BALB/clymphoma cell line L10.A2J was fused to T cell-depleted spleen cells from mouse strains bearing other H-2 haplotypes. A portion of the selected hybrids expressed Ia antigens of the normal spleen cell partner in the fusion as evidenced by their presentation of various antigens to a set of antigen-specific I-region-restricted T cell hybridomas. Three cloned hybrids were studied in detail. Antigen presentation was inhibited specifically by monoclonal anti-Ia antibodies. Both I-A and I-E molecules were expressed, including in the one case examined hybrid I-A and I-E molecules between the H-2d and H-2b haplotypes. These Ia+ B cell hybridomas provide a useful set of tools for studying the role of I-region-encoded molecules in antigen presentation to T cells.