Microfluidic Encapsulation of Ovarian Follicles for 3D Culture.
Microfluidic Encapsulation of Ovarian Follicles for 3D Culture.
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DOI：
10.1007/s10439-017-1823-7
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发表时间：
2017-07
影响因子：
3.8
通讯作者：
He X
中科院分区：
文献类型：
--
作者：
He X
卵泡是哺乳动物卵巢最基本的功能组织单位，含有一个卵母细胞。因此，分离和体外培养卵泡以获得可受精的卵母细胞被认为是妇女对抗不孕症的有希望的策略。本文对近年来国内外关于微流控技术在核壳型水凝胶微囊中包裹卵泡进行仿生三维培养的研究进展进行了综述。这些研究强调了卵巢细胞外基质的机械异质性的重演对于体外培养早期腔前卵泡发育到腔期以及体外从腔卵泡释放卵丘-卵母细胞复合物（COC）至关重要。基于水凝胶的培养系统和微流体技术可能是无价的，以促进卵泡培养作为一个可行的选择，恢复妇女的生育能力在临床上。
The ovarian follicle that contains one single oocyte is the fundamental functional tissue unit of mammalian ovary. Therefore, isolation and in vitro culture of ovarian follicles to obtain fertilizable oocytes are regarded as a promising strategy for women to combat infertility. In this communication, we performed a brief survey of studies on microfluidic encapsulation of ovarian follicles in core-shell hydrogel microcapsules for biomimetic 3D culture. These studies highlighted that recapitulation of the mechanical heterogeneity of the extracellular matrix in ovary is crucial for in vitro culture to develop early pre-antral follicles to the antral stage, and for the release of cumulus-oocyte complex (COC) from antral follicles in vitro. The hydrogel encapsulation-based culture system and the microfluidic technology may be invaluable to facilitate follicle culture as a viable option for restoring women’s fertility in the clinic.