Environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters
Environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters
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DOI：
10.1111/j.1365-2672.2010.04701.x
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发表时间：
2010-08-01
影响因子：
4
通讯作者：
Gin, K. Y-H.
中科院分区：
文献类型：
--
作者：
Aw, T. G.;Gin, K. Y-H.
目的：研究热带地区城市污水中的水传播病原病毒的流行情况和基因型。方法和结果：采用分子生物学方法对新加坡3个再生水厂的污水中的病毒进行了研究。在6个月的采样期内，在100%的污水和二级出水中检测到腺病毒、星状病毒和诺如病毒基因组I（GI）和II（GII）。肠道病毒和甲型肝炎病毒（HAV）被发现在94%和78%的污水，89%和28%的二级出水，分别。通过使用定量实时PCR，估计污水中的星状病毒的浓度比腺病毒、诺如病毒GI和GII高1-2个数量级。环境分离株的基因分型显示GI和GII诺如病毒的多种基因型。柯萨奇病毒A型、星状病毒1型和腺病毒41型是流行的。诺如病毒GII/4和柯萨奇病毒A24分离株在废水中密切相关的各自的爆发菌株分离之前在新加坡。结论：这项研究表明，广泛发生的所有测试肠道病毒群在城市废水。热带地区的星状病毒，肠道病毒和诺如病毒的遗传多样性observed.Significance和影响的研究：在城市污水中的人类肠道病毒的高流行率和巨大的遗传多样性，强烈支持需要进一步全面的研究，以评估与病毒病原体在水环境中的公共卫生风险。
Aims:To study the prevalence and genotypes of waterborne pathogenic viruses in urban wastewaters in the tropical region.Methods and Results:Viruses in wastewaters collected at three water reclamation plants in Singapore were studied by molecular methods. Over a 6-month sampling period, adenoviruses, astroviruses and both norovirus genogroups I (GI) and II (GII) were detected in 100% of the sewage and secondary effluent. Enteroviruses and hepatitis A viruses (HAV) were found in 94 and 78% of sewage, and 89 and 28% of secondary effluent, respectively. By using quantitative real-time PCR, estimated concentrations of astrovirus in the sewage were 1-2 orders of magnitude higher than those for adenovirus, noroviruses GI and GII. Genotyping of environmental isolates revealed multiple genotypes of GI and GII noroviruses. Coxsackieviruses A, astrovirus type 1 and adenovirus type 41 were prevalent. Norovirus GII/4 and coxsackievirus A24 isolates in wastewaters were closely related to respective outbreak strains isolated previously in Singapore.Conclusions:This study showed the widespread occurrence of all tested enteric virus groups in urban wastewaters. Genetic diversity of astroviruses, enteroviruses and noroviruses in the tropical region was observed.Significance and Impact of the Study:The high prevalence and great genetic diversity of human enteric viruses in urban wastewaters strongly supports the need of further comprehensive studies for evaluating the public health risk associated with viral pathogens in water environments.