Genetics of symbiosis in Lotus japonicus:: Recombinant inbred lines, comparative genetic maps, and map position of 35 symbiotic loci
Genetics of symbiosis in Lotus japonicus:: Recombinant inbred lines, comparative genetic maps, and map position of 35 symbiotic loci
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DOI：
10.1094/mpmi-19-0080
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发表时间：
2006-01-01
影响因子：
3.5
通讯作者：
Stougaard, J
中科院分区：
文献类型：
--
作者：
Sandal, N;Petersen, TR;Stougaard, J
用于分析植物遗传对根瘤菌和菌根共生的贡献的分子工具的开发为我们对植物-微生物相互作用的理解提供了重大进展，并且已经鉴定和表征了几个关键的共生基因。为了提高豆科植物百脉根模型的遗传分析效率，我们在此提出了一系列改进的遗传工具。先前通过一组锚定标记比对从L. japonicus Gifu和L. filicaulis之间的种间杂交以及两种生态型L. japonicus Gifu和L. japonicus MG-20之间的种内杂交开发的两个遗传连锁图谱。突出显示了连锁群的区域，其中优先使用一种或另一种亲本组合获得遗传分辨率。在由两个群体的单种子血统衍生的重组自交系中获得了额外的遗传分辨率和稳定的作图群体。为了更快地绘制新基因座，选择分布在染色体臂上的可靠标记提供了一个通用框架，可以更有效地识别未表征的突变系中的新等位基因和新共生基因座。结合莲花社区的资源，提供了大量共生基因座的图谱位置以及等位基因和紧密连锁的分子标记。总而言之，这为莲花属建立了共同的遗传资源。基于网络的版本将使该资源能够定期管理和更新。
Development of molecular tools for the analysis of the plant genetic contribution to rhizobial and mycorrhizal symbiosis has provided major advances in our understanding of plant-microbe interactions, and several key symbiotic genes have been identified and characterized. In order to increase the efficiency of genetic analysis in the model legume Lotus japonicus, we present here a selection of improved genetic tools. The two genetic linkage maps previously developed from an interspecific cross between L. japonicus Gifu and L filicaulis, and an intraspecific cross between the two ecotypes L. japonicus Gifu and L. japonicus MG-20, were aligned through a set of anchor markers. Regions of linkage groups, where genetic resolution is obtained preferentially using one or the other parental combination, are highlighted. Additional genetic resolution and stabilized mapping populations were obtained in recombinant inbred lines derived by a single seed descent from the two populations. For faster mapping of new loci, a selection of reliable markers spread over the chromosome arms provides a common framework for more efficient identification of new alleles and new symbiotic loci among uncharacterized mutant lines. Combining resources from the Lotus community, map positions of a large collection of symbiotic loci are provided together with alleles and closely linked molecular markers. Altogether, this establishes a common genetic resource for Lotus spp. A web-based version will enable this resource to be curated and updated regularly.