RECONSTITUTION OF I-KATP - AN INWARD RECTIFIER SUBUNIT PLUS THE SULFONYLUREA RECEPTOR
RECONSTITUTION OF I-KATP - AN INWARD RECTIFIER SUBUNIT PLUS THE SULFONYLUREA RECEPTOR
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DOI：
10.1126/science.270.5239.1166
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发表时间：
1995-11-17
期刊：
影响因子：
56.9
通讯作者：
BRYAN, J
中科院分区：
文献类型：
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作者：
INAGAKI, N;GONOI, T;BRYAN, J
在这里克隆了一个内纠偏钾通道家族的成员。该通道被称为BIR (Kir6.2)，在大鼠胰岛和葡萄糖反应性胰岛素分泌细胞系中大量表达。与磺酰脲受体SUR的共表达重组了76皮西特的内整流钾电导，该电导对三磷酸腺苷（ATP） （I-KATP）敏感，并被磺酰脲类药物抑制，被二氮氧化合物激活。这些数据表明，这些胰腺细胞钾通道是由至少两个亚基组成的复合物，bir是内向整流钾通道家族的成员，SUR是atp结合盒超家族的成员。基因定位数据显示，这两个钾通道亚基基因聚集在人类11号染色体11p15.1位置。
A member of the inwardly rectifying potassium channel family was cloned here. The channel, called BIR (Kir6.2), was expressed in large amounts in rat pancreatic islets and glucose-responsive insulin-secreting cell lines. Coexpression with the sulfonylurea receptor SUR reconstituted an inwardly rectifying potassium conductance of 76 picosiemens that was sensitive to adenosine triphosphate (ATP) (I-KATP) and was inhibited by sulfonylureas and activated by diazoxide. The data indicate that these pancreatic beta cell potassium channels are a complex composed of at least two subunits-BIR, a member of the inward rectifier potassium channel family, and SUR, a member of the ATP-binding cassette superfamily. Gene mapping data show that these two potassium channel subunit genes are clustered on human chromosome 11 at position 11p15.1.