A One-stage Factor VIII (Antihaemophilic Globulin) Assay and its Use on Venous and Capillary Plasma
A One-stage Factor VIII (Antihaemophilic Globulin) Assay and its Use on Venous and Capillary Plasma
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一阶段因子 VIII（抗血友病球蛋白）测定及其在静脉和毛细血管血浆中的应用
DOI：
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发表时间：
1962
影响因子：
6.7
通讯作者：
J. Macpherson
中科院分区：
文献类型：
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作者：
R. Hardisty;J. Macpherson
本文以Lang-Dell，Wagner和Brinkhous(1953)的方法为基础，提出了一种通过向体系中加入高岭土悬浮液来控制接触活化的一步法。实验表明，这种修改对凝血时间和系统的重复性的影响。该方法的误差用一系列随机的野外测试的置信限来表示，这些置信限介于-156%和76-131%的观测结果之间。用这种方法对116名正常人的静脉血浆样本测定的凝血因子-VIII浓度的正常范围是50-170%，大致呈正态分布。对110份血浆样品同时进行的一段和两段凝血因子-VIII检测结果之间，以及一系列正常和血友病血浆混合物的凝血因子-VIII的观察浓度和预期浓度之间有很好的相关性。介绍了该方法在毛细血管血浆样品上的应用，并与静脉血浆的结果进行了比较。讨论了毛细血管第八因子测定的实用价值。
Summary A one-stage factor-VIII assay is described, based on the method of Lang-dell, Wagner and Brinkhous (1953), in which contact activation is controlled by the addition of a suspension of kaolin to the system. Experiments to show the effect of this modification on the clotting times and reproducibility of the system are presented. The error of the method is expressed in terms of the confidence limits of a random series of field assays, which lay between 64—156% and 76—131% of the observed result. The normal range of factor-VIII concentrations, as determined by this method on venous plasma samples from 116 normal subjects, was 50—170%, with a roughly normal distribution. There was good correlation between the results of one-stage and two-stage factor-VIII assays carried out in parallel on 110 plasma samples and between the observed and expected factor-VIII concentrations of a series of mixtures of normal and haemophilic plasma. The use of the assay on capillary plasma samples is described, and the results compared with those obtained on venous plasma. The practical value of capillary factor-VIII assays is discussed.