An orthotopic colon cancer model for studying the B7-H3 antitumor effect in vivo
An orthotopic colon cancer model for studying the B7-H3 antitumor effect in vivo
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DOI：
10.1016/j.gassur.2006.02.001
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发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
Encke, Jens
中科院分区：
文献类型：
--
作者：
Lupu, Catalin M.;Eisenbach, Christoph;Encke, Jens
我们建立了小鼠结肠癌原位动物模型，并应用该模型研究 B7 共刺激分子家族的最新成员 B7-H3 的抗肿瘤作用。将Colon-26小鼠结肠腺癌细胞接种到小鼠的盲肠浆膜下层以诱导结肠肿瘤生长。观察小鼠肿瘤生长速度及生存时间。建立稳定转染B7-H3的Colon-26细胞系并研究其免疫原性效果。所有植入野生型肿瘤细胞的小鼠结肠中都有肿瘤生长并死亡。平均存活率为 23 天。植入C26-B7-H3的小鼠的存活时间显着延长至38天。我们的数据表明，B7-H3 对结肠腺癌具有抗肿瘤作用，可考虑作为结肠癌治疗的辅助免疫疗法。我们的小鼠结肠癌原位动物模型可应用于结肠癌的体内实验研究。
We established an orthotopic animal model of colon cancer in mice and applied this model to study the antitumor effects of B7-H3, the newest member of the B7 family of costimulatory molecules. Colon-26 murine colon adenocarcinoma cells were inoculated into the cecal subserosum of mice to induce colon tumor growth. The tumor growth rate and the survival time of the mice were observed. A stable B7-H3 transfected Colon-26 cell line was established and the immunogenic effect was investigated. All mice implanted with wild-type tumor cells had tumor growth in the colon and died. The mean survival rate was 23 days. Mice implanted with C26-B7-H3 had a significantly prolonged survival time of 38 days. Our data suggest that B7-H3 exerts an antitumor effect on adenocarcinoma of the colon and may be considered as an adjuvant immunotherapy in the treatment of colon cancers. Our orthotopic animal model of colon cancer in mice could be applied to in vivo experimental studies of colon cancer.