Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells.

Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells.
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可分离双层微滤装置,用于无标记富集活循环肿瘤细胞。

DOI:
10.1007/978-1-4939-7144-2_6
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发表时间:
2017
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Zheng,Si-Yang
Zheng,Si-Yang
中科院分区:
--
文献类型:
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作者:
Hao,Sijie;Nisic,Merisa;He,Hongzhang;Tai,Yu-Chong;Zheng,Si-Yang

文献摘要

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Analysis of rare circulating tumor cells enriched from metastatic cancer patients yields critical information on disease progression, therapy response, and the mechanism of cancer metastasis. Here we describe in detail a label-free enrichment process of circulating tumor cells based on its unique physical properties (size and deformability). Viable circulating tumor cells can be successfully enriched and analyzed, or easily released for further characterization due to the novel separable two-layer design.