Isolation and quantification of tuliposides and tulipalins in tulips (Tulipa) by high-performance liquid chromatography
Isolation and quantification of tuliposides and tulipalins in tulips (Tulipa) by high-performance liquid chromatography
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DOI：
10.1111/j.1600-0536.1999.tb06080.x
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发表时间：
1999-06-01
影响因子：
5.5
通讯作者：
Kristiansen, K
中科院分区：
文献类型：
--
作者：
Christensen, LP;Kristiansen, K
以水-甲醇梯度为流动相，采用反相高效液相色谱法测定郁金香属植物和栽培品种中郁金香皂苷和郁金香皂苷的含量。采用二极管阵列检测器，检测波长为208 nm。研究表明，除1-和6-郁金香苷A、6-郁金香苷D和内酯化的苷元郁金香苷A和(-)-郁金香苷B外，还有新的郁金香苷F和6-郁金香苷B，后者是已知的1-郁金香苷B的新的酰基衍生物。主要成分为6-郁金香苷A和6-郁金香苷B，含量分别高达鲜重的1.5%和1.3%。6-郁金香苷A和郁金香苷A似乎是郁金香中的主要过敏原，尽管郁金香中的郁金香苷D和F也可能与过敏特性有关。郁金香红素A和(-)-郁金香红素B存在于完整的郁金香中，并且不仅是在真菌侵袭或植物切除后产生的。少数品种的过敏原含量很低，而郁金香苷B的含量相对较高，这表明培育出不会过敏和抗病的郁金香是有可能的。
The content of tuliposides and tulipalins were determined in Tulipa species and cultivars by reversed-phase high-performance liquid chromatography (RP-HPLC)I using a water:methanol gradient as mobile phase. The compounds were detected by a diode array detector employed at 208 nm. The investigation revealed, in addition to 1- and 6-tuliposide A. tuliposide D and the lactonized aglycones tulipalin A and (-)-tulipalin B, the new tuliposide F and 6-tuliposide B, the latter being a new acyl derivative of the known 1-tuliposide B. All compounds were isolated by preparative RP-HPLC and identified by NMR and mass spectroscopy. The predominant compounds were 6-tuliposide A and B present in amounts up to 1.5% and 1.3% of fresh weight, respectively. 6-Tuliposide A and tulipalin A seem to be the major allergens in tulips, although tuliposide D and F may also contribute to the allergenic properties. Tulipalin A and (-)-tulipalin B occur in intact tulips and are not only produced in response to fungal attack or after excision of the plants. A few species were found to have very low allergen content and a relatively high level of tuliposide B, indicating it should be possible to breed non-allergenic and disease-resistant tulips.