The bacterial community structures in response to the gut passage of earthworm (Eisenia fetida) feeding on cow dung and domestic sludge: Illumina high-throughput sequencing-based data analysis
The bacterial community structures in response to the gut passage of earthworm (Eisenia fetida) feeding on cow dung and domestic sludge: Illumina high-throughput sequencing-based data analysis
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以牛粪和生活污泥为食的蚯蚓 (Eisenia fetida) 肠道通过的细菌群落结构：Illumina 基于高通量测序的数据分析
DOI：
10.1016/j.ecoenv.2019.110149
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发表时间：
2020-03-01
影响因子：
6.8
通讯作者：
Kang, Yijun
中科院分区：
文献类型：
--
作者：
Hu, Jian;Zhao, Haitao;Kang, Yijun
饮食被证明能够塑造肠道微生物群，而不同食物对不同消化道细菌群落的影响尚不清楚。为此，牛粪（CD）和生活污泥（DS）被选为蚯蚓（蚯蚓）的饲料，不同的肠道内容物，即砂囊+前肠面积，后肠，和成熟的蠕虫堆肥取样Illumina测序分析。结果表明，在砂囊+前肠区，细菌多样性和丰度显著降低，但存在稳定的具有生物降解能力的细菌，如拟无枝酸菌属（Amycolatopsis）、甲基杆菌属（Methylobacterium）、罗尔斯通氏菌属（Ralstonia）、苍白杆菌属（Ochrobactrum）和鞘氨醇单胞菌属（Sphingomonas）。后肠和成熟蚯蚓堆肥中的减少量可以不同程度地恢复，表明胃+前肠区域对细菌群落产生了瓶颈效应。β-变形菌是最丰富的亚类，无论不同的饮食，和属于伽马，δ-和ε-亚类的细菌作为食物的蚯蚓。根据不同的目的，应根据不同的饲料使用不同的蚯蚓堆肥。
Diets are shown to be capable of shaping the gut microbiota of earthworm, while the effects of distinct foods on bacterial communities of different digestive tracts of earthworm are unknown. For this purpose, cow dung (CD) and domestic sludge (DS) were chosen as diets for earthworms (Eisenia fetida), and different gut contents, namely gizzard + foregut area, hindgut, and mature vermi-compost were sampled for Illumina sequencing analysis. We found that there existed significant reductions in bacterial diversity and abundance in the gizzard + foregut area, where there were stable bacteria with the ability of biodegradation of xenobiotics, such as Amycolatopsis, Methylobacterium, Ralstonia, Ochrobactrurn, and Sphingomonas. The decreases could be recovered in the hindgut and mature vermi-compost to different extents, suggesting that a bottleneck effect on the bacterial community occurred in the gizzard + foregut area. Beta-Proteobacteria was the most abundant subclass regardless of the different diets, and bacteria affiliated with gamma-, delta- and epsilon-subclasses were taken as food by the earthworms. Vermi-composts based on the various diets should be used differently according to different aims.