Genetic Approaches for Signaling Pathways and Proteins
Genetic Approaches for Signaling Pathways and Proteins
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信号通路和蛋白质的遗传学方法
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
J. S. Parkinson
中科院分区：
文献类型：
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作者：
J. S. Parkinson
本章讨论了一些遗传学的方法来研究信号通路和阐明模块化信号蛋白的信息处理的分子机制。细菌生活在不稳定的环境中。营养和毒素水平，酸度，温度，渗透压，湿度和许多其他条件可以迅速和意想不到的变化。细菌的信号传导系统可以进行详细的遗传和生化分析。在这一章中，一些一般的策略，利用遗传学的方法来研究感觉通路和信号蛋白进行了讨论。许多来自革兰氏阳性和革兰氏阴性细菌的信号蛋白含有特征性的“发射器”和“接收器”结构域，其促进蛋白质内和蛋白质之间的信息传递。发射器和接收器非常适合作为组装信号通路的电路元件。唯一被证实的发射器-接收器通信机制涉及磷酸化和去磷酸化反应。谨慎地说，上位性分析的逻辑可以扩展到更复杂的信号通路，如分支、反馈环等，遗传学方法可以为信号通路和蛋白质的运作提供相当多的见解。尽管实际的信号电路不太可能像本章中的两个元件示例那么简单，但应该适用相同的基本原则。
This chapter discusses some genetic approaches for studying signaling pathways and for elucidating the molecular mechanisms of information processing by modular signaling proteins. Bacteria live in precarious environments. Nutrient and toxin levels, acidity, temperature, osmolarity, humidity, and many other conditions can change rapidly and unexpectedly. Bacterial signaling systems are amenable to detailed genetic and biochemical analyses. In this chapter, some general strategies for using genetic methods to study sensory pathways and signaling proteins are discussed. Many signaling proteins, from both gram-positive and gram-negative bacteria, contain characteristic "transmitters" and "receivers" domains that promote information transfer within and between proteins. Transmitters and receivers are ideally suited as circuit elements for assembling signaling pathways. The only demonstrated mechanisms of transmitter-receiver communication involve phosphorylation and dephosphorylation reactions. With caution, the logic of epistatic analysis can be extended to more elaborate signaling pathways that have branches, feedback loops, and so on. Genetic approaches can provide considerable insight into the operation of signaling pathways and proteins. Even though actual signaling circuits are unlikely to be as simple as the two-component examples in the chapter, the same basic principles should apply.
影响因子：
56.9
作者：
UTSUMI, R;BRISSETTE, RE;INOUYE, M
通讯作者：
INOUYE, M