Erythrocytes may synthesize their own nitric oxide
Erythrocytes may synthesize their own nitric oxide
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DOI：
10.1016/s0895-7061(96)00257-9
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发表时间：
1996-12-01
影响因子：
3.2
通讯作者：
Gierman, JL
中科院分区：
文献类型：
--
作者：
Jubelin, BC;Gierman, JL
血红蛋白和肌红蛋白结合由其他细胞和循环血管扩张剂产生的一氧化氮（NO）的能力是众所周知的。为了表征红细胞（RBC），我们使用NADPH黄递酶标记，以及三种已知类型的NO合酶（NOS 1，2和3）的抗体，以及通常与NOS相关的分子，钙调素。我们表明，NADPH-心肌黄酶标签标签肌间神经元，内皮细胞，和血细胞被困在管腔中的血管贯穿肠壁。肌间神经元也呈神经元型NOS（NOS 1）、钙调素和神经肽Y阳性，表明它们是产生NO的神经元。内皮细胞对NOS 3（NOS的组成型）呈阳性，而巨噬细胞和淋巴细胞对NOS 2（NOS的诱导型）呈阳性。都是钙调素阳性。令人惊讶的是，红细胞对NOS 2和3以及钙调蛋白呈阳性。因此，红细胞拥有所有的细胞机制来合成自己的NO。我们认为，红细胞会合成和使用NO来调节自己的生理。(C)1996年美国高血压杂志有限公司
The ability of hemoglobin and myoglobin to bind nitric oxide (NO) produced by other cells and circulating vasodilators is well known. To characterize erythrocytes (RBCs), we used NADPH diaphorase labeling, as well as antibodies to the three known types of NO synthase (NOS 1, 2, and 3), and to a molecule usually associated with NOS, calmodulin. We show that the NADPH-diaphorase label labels myenteric neurons, endothelial cells, and the blood cells trapped in the lumen of the blood vessels running through the intestinal wall. The myenteric neurons are also positive for neuronal NOS (NOS1), calmodulin, and neuropeptide Y, indicating that they are NO-producing neurons. Endothelial cells are positive for NOS3 (a constitutive form of NOS), while macrophages and lymphocytes are positive for NOS2 (an inducible form of NOS). All are positive for calmodulin. Surprisingly, the RBCs are positive for NOS2 and 3, as well as calmodulin. Thus the RBCs possess all the cellular machinery to synthesize their own NO. We suggest that erythrocytes would synthesize and use NO to modulate their own physiology. (C) 1996 American Journal of Hypertension, Ltd.