Measurement of incident angle dependence of swimming bacterium reflection using on-chip single-cell cultivation assay
Measurement of incident angle dependence of swimming bacterium reflection using on-chip single-cell cultivation assay
复制标题
DOI：
10.1143/jjap.42.l873
复制
发表时间：
2003-07-15
期刊：
影响因子：
--
通讯作者：
Yasuda, K
中科院分区：
文献类型：
--
作者：
Hattori, A;Umehara, S;Yasuda, K
我们开发了一种片上单细胞微培养测定法，作为连续观察某些单个游泳细胞以追踪其运动的手段。单细胞被捕获在薄载玻片上制成的微室中，并具有半透膜盖，细胞可以在其中长期游动而不会逃逸。该测定能够根据某些细胞的入射角直接测量某些细胞对微室壁的反射。使用该测定，检查反射。我们发现，尽管大多数细胞在入射角垂直于壁时发生反射，但细胞的反射率与非反向细胞的反射率几乎相同。
We have developed an on-chip single-cell microcultivation assay as a means of continuously observing certain single swimming cells in order to trace their movement. The single cells were captured in microchambers fabricated on thin glass slides and having semipermeable membrane lids, in which cells can swim within the space for a long term without escaping. This assay enables the direct measurement of the reflection of certain cells against the microchamber walls depending on their incidence angles. Using this assay, the reflection was examined. We found that the ratio of reflection of cells to those of non-reverse was almost the same, though most of cells reflected when their incident angle was perpendicular to the wall.