Directed evolution of enzyme catalysts
Directed evolution of enzyme catalysts
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DOI：
10.1016/s0167-7799(97)01138-4
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发表时间：
1997-12-01
影响因子：
17.3
通讯作者：
Arnold, FH
中科院分区：
文献类型：
--
作者：
Kuchner, O;Arnold, FH
定向酶进化在过去几年中出现，成为工程生物催化剂合理方法的有力替代方案。成功定向进化的先决条件是在合适的微生物宿主中进行功能表达、快速筛选所需特征以及深思熟虑的蛋白质景观工作策略。关于酶体外进化的文献数量迅速增长，包括创建和搜索组合酶库的技术，以及使用不同进化策略的几个成功例子。
Directed enzyme evolution has emerged in the past few years as a powerful alternative to rational approaches for engineering biocatalysts. Prerequisites for successful directed evolution are functional expression in a suitable microbial host, a rapid screen for the desired feature(s) and a well-thought-out working strategy for navigating protein landscapes. The rapidly growing body of literature on enzyme evolution in vitro includes techniques for creating and searching combinatorial enzyme libraries, as well as several successful examples of different evolutionary strategies being used.