Application of a new bioassay technique using goldfish for assessment of water toxicity
Application of a new bioassay technique using goldfish for assessment of water toxicity
复制标题
DOI：
10.1002/tox.20379
复制
发表时间：
2008-12
影响因子：
4.5
通讯作者：
Y. Deguchi;Nan Wu;Tomoyasu Toyoizumi;S. Masuda;H. Nagaoka;Tetsushi Watanabe;Y. Totsuka;K. Wakabayashi;N. Kinae
中科院分区：
文献类型：
--
作者：
Y. Deguchi;Nan Wu;Tomoyasu Toyoizumi;S. Masuda;H. Nagaoka;Tetsushi Watanabe;Y. Totsuka;K. Wakabayashi;N. Kinae
水环境中的化学物质种类繁多，对其进行毒性评价具有重要意义。生物标记物如DNA损伤诱导、微核、卵黄蛋白原和肝脏P450等已知可有效监测遗传毒性和/或雌激素化学品。然而，很少有研究将这些生物标志物用于同一种鱼。金鱼（Carassius auratus）被广泛使用，大小适合收集血液或器官。在这项研究中，使用标准化学品检查金鱼中多种生物标志物的有效性，并将其应用于河水中。据报告，流经日本静冈县纺织印染厂的Ho河对鼠伤寒沙门氏菌TA 98和YG 1024显示出遗传毒性。当金鱼暴露于河，DNA损伤，雌激素活性，和CYP 1A诱导观察。通过这项研究，我们假设，不仅诱变剂/致癌物，而且内分泌干扰化学品和多环芳烃存在于河。因此，应要求进行化学鉴别。金鱼可以同时评价遗传毒性和雌激素活性，是评价水环境中化学污染水平的良好实验模型。© 2008 Wiley Periodicals，Inc.环境毒理学，2008年。
There are a variety of chemicals in aquatic environment, so it is important to assess the toxicity. The biomarkers such as induction of DNA damage, micronuclei, vitellogenin, and hepatic P450 in fish are known to be effective for monitoring genotoxic and/or estrogenic chemicals. However, there is little study to use these biomarkers in same fish. Goldfish (Carassius auratus) is widely used and is suitable in size to collect blood or organs. In this study, validity of multiple‐biomarkers in goldfish was checked using standard chemicals and applied in the river water. Ho River, which flows through the textile dyeing factory in Shizuoka Prefecture, Japan, was reported to show genotoxicity toward Salmonella typhimurium TA98 and YG1024. When the goldfish were exposed to Ho River, DNA damage, estrogenic activity, and CYP1A induction were observed. Through the study, it was assumed that not only mutagens/carcinogens but also endocrine disrupting chemicals and poly aromatic hydrocarbons were present in Ho River. Therefore, chemical identification should be required. We could evaluate both genotoxicity and estrogenic activity simultaneously, so goldfish might be a good experimental model for estimation of chemical contamination levels in aquatic environment. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.