Absorption activity and barrier properties in the endolymphatic sac. Ultrastructural and morphometric analysis.
Absorption activity and barrier properties in the endolymphatic sac. Ultrastructural and morphometric analysis.
复制标题
内淋巴囊的吸收活性和屏障特性。
作者：
H. Hoshikawa;H. Furuta;N. Mori;S. Sakai
将一定体积的辣根过氧化物酶（HRP）直接注射到豚鼠的内淋巴囊（ES）腔中，以研究ES的详细吸收活性和屏障特性。反应产物进行了分析，使用超微结构和形态测定方法1至10小时后，注射这种示踪剂。近端部分的上皮细胞在示踪剂注射后的任何时间间隔都不吸收管腔内HRP。中间部分的上皮细胞根据它们的吸收活性被清楚地分为两种类型：活性吸收细胞和非活性细胞。主动吸收细胞的摄取在注射后8h达到最大，然后下降。中间部的活性吸收细胞被认为在ES上皮的大分子吸收中起主要作用。非活性细胞几乎不吸收管腔内HRP，表明这些细胞不参与大分子吸收。注射HRP后1 ~ 8h，远侧部的吸收活性低于中间部的吸收活性细胞，而注射后10 h，远侧部的吸收活性高于中间部的吸收活性细胞。不仅中间部分，而且远端部分在大分子吸收中也可发挥积极作用。在没有一部分的ES没有管腔内的HRP渗透超过上皮细胞之间的连接复合物或通过细胞质。可以想象，在ES的上皮衬里中存在对管腔内大分子的紧密屏障。
A constant volume of horseradish peroxidase (HRP) was injected directly into the endolymphatic sac (ES) lumen of the guinea pig to investigate the detailed absorption activity and the barrier properties of the ES. The reaction products were analyzed using an ultrastructural and morphometric method 1 to 10 h after the injection of this tracer. The epithelial cells in the proximal portion did not absorb the intraluminal HRP at any intervals after the tracer injection. The epithelial cells of the intermediate portion were classified clearly into two types according to their absorption activity: active-absorptive, and non-active cells. Uptake in the active-absorptive cells reached its maximal rate 8 h after the injection and then decreased. The active-absorptive cells in teh intermediate portion are considered to play a major role in the macromolecular absorption in the ES epithelium. The non-active cells scarcely absorbed the intraluminal HRP, suggesting that these cells are not involved in the macromolecular absorption. The absorption activity in the distal portion was lower than that in the active-absorptive cells in the intermediate portion 1 to 8 h after the HRP injection, while higher 10 h after the injection. Not only the intermediate portion but also the distal portion may play an active role in the macromolecular absorption. In no portion of the ES did intraluminal HRP penetrate beyond the junctional complexes between epithelial cells or through the cytoplasm. It is conceivable that there is a tight barrier to the intraluminal macromolecules in the epithelial linings of the ES.
内耳的实验免疫系统。
DOI：
10.1159/000275942
发表时间：
1987
期刊：
ORL; journal for oto-rhino-laryngology and its related specialties
影响因子：
--
作者：
Harris,JP;Tomiyama,S
通讯作者：
Tomiyama,S