Low levels of genetic variation in mtDNA sequences over the western Mediterranean and Atlantic range of the sponge Crambe crambe (Poecilosclerida)
Low levels of genetic variation in mtDNA sequences over the western Mediterranean and Atlantic range of the sponge Crambe crambe (Poecilosclerida)
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DOI：
10.1007/s00227-003-1178-5
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发表时间：
2004-01-01
期刊：
影响因子：
2.4
通讯作者：
Turon, X
中科院分区：
文献类型：
--
作者：
Duran, S;Pascual, M;Turon, X
海甘蓝是一种常见的结壳海绵，发现于地中海和大西洋沿岸的。本文对该物种线粒体DNA（mtDNA）基因细胞色素氧化酶亚基I（COI）的部分序列（535 bp）进行了分析，试图确定该物种的种群结构。这是该门动物中首次利用这种标记进行群体遗传学研究。样本（N=86）取自8个种群，距离从20到3,000公里，从西地中海到大西洋。发现该基因的低变异性，因为仅鉴定出两种单倍型，沿着具有低核苷酸多样性（pi=0.0006）。然而，不同的频率之间发现的种群揭示了遗传结构和低基因流之间的密切人群，从物种的扩散生物学的预期。海绵中发现的低变异性与刺胞动物的报告一致，并指出线粒体DNA在双胚层门中的高度保守性。
Crambe crambe is a common encrusting sponge found in the Mediterranean and Atlantic littoral. An analysis of a partial sequence (535 bp) of the mitochondrial DNA (mtDNA) gene cytochrome oxidase subunit I (COI) was conducted in an attempt to determine population structure in this species. This is the first study of population genetics using this kind of marker in the phylum. Samples (N=86) were taken in eight populations separated by distances from 20 to 3,000 km, spanning from the western Mediterranean to the Atlantic. Low variability of this gene was found, as only two haplotypes were identified, along with low nucleotide diversity (pi=0.0006). However, the different frequencies found among populations revealed genetic structure and low gene flow between close populations, as expected from the dispersal biology of the species. The low variability found in sponges is in agreement with reports on cnidarians and points to a high conservation of mtDNA in diploblastic phyla.