Phylogeny of Panax using chloroplast trnC-trnD intergenic region and the utility of trnC-trnD in interspecific studies of plants
Phylogeny of Panax using chloroplast trnC-trnD intergenic region and the utility of trnC-trnD in interspecific studies of plants
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DOI：
10.1016/j.ympev.2003.10.009
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发表时间：
2004-06-01
影响因子：
4.1
通讯作者：
Wen, J
中科院分区：
文献类型：
--
作者：
Lee, C;Wen, J
获得了所有人参属植物叶绿体trnC-trnD区和核核糖体DNA内转录间隔区（ITS）的序列。(the人参属，五加科），以重建系统发育关系。trnC-trnD同源性与人参属二倍体的ITS同源性一致。本研究是首次利用trnC-trnD序列数据在种间水平上进行系统发育分析。我们评估了这个DNA区域的系统发育效用在较低的分类水平显花植物。trnC-trnD区域包括trnC-petN基因间间隔区、petN基因、petN-psbM基因间间隔区、psbM基因和psbM-trnD基因间间隔区。petN和psbM基因很小，在被子植物中分别为90和104-114 bp，并且具有保守序列。我们设计了这两个基因的通用扩增和测序引物。使用这些引物，我们已经成功地扩增了整个trnC-trnD区域的开花植物群体，包括辽东木的多样性。（五加科）、夏蜡梅（Calycanthus L.）（萼科）、榛属（Corylus L.）（桦木科）、金缕梅属（Hamamelis L.）（金缕梅科）、天胡荽属。（伞形科）、冬青属（桐科）、莲属（Nelumbo Adans.（莲科）、Nolana L. ex L.f.（Solanaceae）、Prunus L.（蔷薇科）、省沽油属（Staphylea L.）（省沽油科）。在人参中，trnC-trnD区域提供了与ITS区域相似数量的信息系统发育特征，并且比叶绿体ndhF基因提供了略高数量的信息特征。因此，我们证明了trnC-trnD区域对于开花植物低水平系统发育研究的实用性。(C)2003年爱思唯尔公司All rights reserved.
Sequences of the chloroplast trnC-trnD region and the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA were obtained for all species of Panax L. (the ginseng plant genus, Araliaceae) to reconstruct phylogenetic relationships. The trnC-trnD phylogeny is congruent with the ITS phylogeny for the diploid taxa of Panax. This study is the first use of the trnC-trnD sequence data for phylogenetic analysis at the interspecific level. We evaluated this DNA region for its phylogenetic utility at the lower taxonomic level for flowering plants. The trnC-trnD region includes the trnC-petN intergenic spacer, the petN gene, the petN-psbM intergenic spacer, the psbM gene, and the psbM-trnD intergenic spacer. The petN and psbM genes are small, 90 and 104-114 bp across angiosperms, respectively, and have conserved sequences. We have designed universal amplification and sequencing primers within these two genes. Using these primers, we have successfully amplified the entire trnC-trnD region for a diversity of flowering plant groups, including Aralia L. (Araliaceae), Calycanthus L. (Calycanthaceae), Corylus L. (Betulaceae), Hamamelis L. (Hamamelidaceae), Hydrocotyle L. (Apiaceae), Illigera Blume (Hernandiaceae), Nelumbo Adans. (Nelumbonaceae), Nolana L. ex L.f. (Solanaceae), Prunus L. (Rosaceae), and Staphylea L. (Staphyleaceae). In Panax, the trnC-trnD region provides a similar number of informative phylogenetic characters as the ITS regions and a slightly higher number of informative characters than the chloroplast ndhF gene. We thus demonstrate the utility of the trnC-trnD region for lower-level phylogenetic studies in flowering plants. (C) 2003 Elsevier Inc. All rights reserved.