Proteomic and Phosphoproteomic Analysis at Diapause Initiation in the Cotton Bollworm, Helicoverpa armigera
Proteomic and Phosphoproteomic Analysis at Diapause Initiation in the Cotton Bollworm, Helicoverpa armigera
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棉铃虫 Helicoverpa armigera 滞育起始时的蛋白质组和磷酸蛋白质组分析
DOI：
10.1021/pr100356t
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发表时间：
2010-10-01
影响因子：
4.4
通讯作者：
Xu, Wei-Hua
中科院分区：
文献类型：
--
作者：
Lu, Yu-Xuan;Xu, Wei-Hua
滞育是一个发育停滞期，使物种能够适应不利的条件。许多昆虫物种会降低代谢活动，然后在其生命周期的某个阶段进入滞育。当棉铃虫幼虫在短日照和低温下饲养时，棉铃虫将发生蛹滞育。大脑是滞育决策的重要器官，滞育个体大脑中的一些信号分子被释放到血淋巴中来调节滞育。在这项研究中，我们进行了基于二维凝胶的比较蛋白质组和磷酸蛋白质组分析，以寻找非滞育和滞育的蛹大脑之间差异表达的蛋白质。两组之间共有79个蛋白和23个磷蛋白显示出显着差异，并且MALDI-TOF/TOF MS鉴定出41个蛋白和10个磷蛋白。此外，通过 RT-PCR 或蛋白质印迹分析证实了滞育和非滞育的蛹大脑中的基因表达模式。这些差异表达的蛋白质在滞育起始的早期蛹阶段的代谢变化、应激反应和信号转导途径中发挥作用。因此，这些鉴定出的蛋白质可能会抑制滞育蛹的新陈代谢，导致昆虫进入发育停滞。
Diapause is a period of developmental arrest that allows a species to adapt to unfavorable conditions. Many insect species reduce metabolic activity and then enter diapause at a certain stage in their life cycles. The cotton bollworm, Helicoverpa armigera, will be destined for pupal diapause when larvae are reared under short daylengths and low temperature. The brain is an important organ for diapause decision, and some signaling molecules from the brain of diapause-destined individuals are released into the hemolymph to regulate diapause. In this study, we performed 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins between nondiapause- and diapause-destined pupal brains. A total of 79 proteins and 23 phosphoproteins showed significant differences between these two groups, and 41 proteins and 10 phosphoproteins were identified by MALDI-TOF/TOF MS. Further, gene expression patterns in diapause- and nondiapause-destined pupal brains were confirmed by RT-PCR or Western blot analysis. These differentially expressed proteins act in the metabolic change, stress response, and signal transduction pathways at early pupal stage for diapause initiation. Thus, these identified proteins may depress metabolism in diapause-destined pupae to lead the insect to enter developmental arrest.