Obtaining fresh prostate cancer tissue for research: A novel biopsy needle and sampling technique for radical prostatectomy specimens
Obtaining fresh prostate cancer tissue for research: A novel biopsy needle and sampling technique for radical prostatectomy specimens
复制标题
DOI：
10.1002/pros.20264
复制
发表时间：
2005-09-01
期刊：
影响因子：
2.8
通讯作者：
Bishop, MC
中科院分区：
文献类型：
--
作者：
Walton, TJ;McCulloch, TA;Bishop, MC
背景由于福尔马林固定会降解细胞内核酸，因此优选新鲜或新鲜冷冻的组织样本进行分子分析。根治性前列腺切除术（RP）标本是前列腺癌组织的一个有价值的来源，但依赖于整个器官的病理学处理的标本限制了取样的机会。很少有研究涉及到特定的收获技术，使用椎间盘切除标本。材料和方法。使用专门设计的针对23个连续的新鲜RP标本进行离体活检。采用标准六分仪入路，每侧额外进行一次侧向活检。将每个叶的核心快速冷冻在一起，并由对RP和术前活检病理学不知情的病理学家评估切片。使用t检验和卡方（2）统计检验与术前活检进行比较。11个随机选择的RP标本进一步评估针迹和边缘穿孔的影响。23例标本中检出癌19例，灵敏度为83.6%。平均肿瘤累及率为28.3%，而术前活检为15.6%（P < 0.02）。两组间Gleason总分一致性或肿瘤位置一致性无统计学显著差异。11例中有3例发现针缘穿孔;所有病例均未影响病理评估，但有1例需要进行更深的块切除。离体活检是一种有用的技术，用于在RP标本中获取新鲜组织，同时保留器官形态。专门设计的针和收获技术提供了良好的收益率的癌组织从高比例的抽样切除标本。
BACKGROUND. Fresh or fresh-frozen tissue samples are preferred for molecular profiling as formalin fixation degrades intracellular nucleic acids. Radical prostatectomy (RP) specimens are a valuable source of prostate cancer tissue, but the reliance on whole-organ pathological processing for prognostication limits sampling opportunities. Few studies have addressed specific harvesting techniques using prostatectomy specimens.MATERIALS AND METHODS. Ex vivo biopsies were performed on 23 consecutive fresh RP specimens using a purpose-designed needle. A standard sextant approach was used with an additional lateral biopsy on each side. Cores from each lobe were snap-frozen together and sections assessed by a pathologist blinded to the RP and pre-operative biopsy pathology. Comparison with pre-operative biopsies was performed using the t-test and chi(2) statistical tests. Eleven randomly selected RP specimens were further evaluated for the effects of needle tracks and margin perforation.RESULTS. Cancer was detected in 19 of 23 specimens, giving a sensitivity of 83.6%. The average tumor involvement was 28.3% per section compared with 15.6% for pre-operative biopsies (P < 0.02). There was no statistically significant difference between the groups for either Gleason sum score concordance or tumor location concordance. In 3 of 11 cases, needle margin perforation was identified; in none of the cases did it compromise pathological assessment, although in one case a deeper block resection was required.CONCLUSIONS. Ex vivo biopsy is a useful technique for retrieving fresh tissue whilst preserving organ morphology in RP specimens. The purpose-designed needle and harvesting technique provide good yields of cancer tissue from a high proportion of sampled prostatectomy specimens.