Microcyn™:: a novel super-oxidized water with neutral pH and disinfectant activity
Microcyn™:: a novel super-oxidized water with neutral pH and disinfectant activity
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DOI：
10.1016/j.jhin.2005.04.021
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发表时间：
2005-12-01
影响因子：
6.9
通讯作者：
Gutierrez, AA
中科院分区：
文献类型：
--
作者：
Landa-Solis, C;González-Espinosa, D;Gutierrez, AA
一种新型超氧化水 (SOW) 产品 Microcyn(TM) 已进行体外抗菌测试。和抗病毒活性。评估了这种中性 pH 值 SOW 在纯培养物中培养金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、伤寒沙门氏菌和白色念珠菌的有效性。将 1 毫升（相当于 10(8) 菌落形成单位/mL）的每种微生物置于 9 mL Microcyn 或无菌水中，在室温下处理 30 s。在这些条件下，处理样品中所有病原体的水平发生了 log(10) 降低因子 8。此外，三批 Microcyn 暴露于萎缩芽孢杆菌孢子 5 分钟的测试结果表明，孢子在 2-3 分钟内完全失活（log(10) 减少因子 >4）。 Microcyn 在减少硬表面（玻璃）上人类免疫缺陷病毒 1 (HIV-1) 方面的有效性也根据环境保护局关于杀病毒声明的要求进行了评估。将测试表面在不搅拌的情况下暴露于 Microcyn 5 分钟后，通过细胞病变效应和 MT-2 培养物中 HIV-1 产生的抗原 p24 测量，病毒载量的 Log(10) 减少因子 > 3。还在模拟实验室病毒使用条件下分析了 Microcyn 针对 5 型腺病毒载体的活性。悬浮液。为了提高测试的灵敏度，使用流式细胞仪测量AdGFP感染的细胞发出的荧光。在这些严格条件下暴露于 Microcyn 5 分钟后，病毒载量的 log(10) 减少因子 > 3。这些结果表明，Microcyn 具有广泛的抗菌谱，与酸性 SOWS 相比具有主要优势，包括中性、pH 值、较低的游离活性氯 (51-85 ppm) 和钳保质期（1 年）。 (C) 2005 年医院感染协会。由爱思唯尔有限公司出版。保留所有权利。
A new super-oxidized water (SOW) product, Microcyn(TM), was tested for in vitro antimicrobial. and antiviral activities. The effectiveness of this neutral-pH SOW at kitting Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans in pure culture was evaluated. One millilitre (similar to 10(8) colony-forming units/mL) of each microorganism was subjected to 9 mL Microcyn or sterile water at room temperature for 30 s. Under these conditions, a log(10) reduction factor of 8 in the level of all pathogens occurred in the treatment samples. In addition, results of tests with three batches of Microcyn exposed to Bacillus atrophaeus spores for 5 min demonstrated complete inactivation of the spores within 2-3 min (log(10) reduction factor >4). The effectiveness of Microcyn in reducing human immunodeficiency virus-1 (HIV-1) on hard surfaces (glass) was also evaluated in compliance with Environmental Protection Agency requirements for virucidal claims. After exposure of the tested surfaces to Microcyn for 5 min without agitation, there was a Log(10) reduction factor > 3 in the viral, load as measured by both cytopathic effect and antigen p24 of HIV-1 production in MT-2 cultures. Microcyn activity against adenoviral vector type 5 was also analysed under simulated Laboratory in-use conditions with viral. suspensions. In order to increase the sensitivity of the test, the fluorescent light emitted by AdGFP-infected cells was measured with the use of a flow cytometer. A log(10) reduction factor > 3 in the viral load was achieved after a 5-min exposure to Microcyn under these strict conditions. These results show that Microcyn exerts a wide antimicrobial spectrum with major advantages over acidic SOWS, including neutral, pH, Lower free active chlorine (51-85 ppm) and tong shelf Life (1 year). (C) 2005 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.