Electrochemical immunosensor for interferon-gamma based on disposable ITO detector and HRP-antibody-conjugated nano gold as signal tag
Electrochemical immunosensor for interferon-gamma based on disposable ITO detector and HRP-antibody-conjugated nano gold as signal tag
复制标题
基于一次性ITO检测器和HRP抗体偶联纳米金作为信号标签的干扰素γ电化学免疫传感器
DOI：
10.1016/j.msec.2015.10.066
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Cai Xiang
中科院分区：
文献类型：
--
作者：
Zhang Yaru;Zhang Bin;Ye Xiaoli;Yan Yuqi;Huang Langhuan;Jiang Zhenyou;Tan Shaozao;Cai Xiang
结核病是全世界与感染相关的死亡最常见的原因。提出了一种成本低、制作简单的检测干扰素-γ(干扰素-γ)的一次性电化学免疫传感器。利用二烯丙基二甲基氯化铵(PDDA)和金纳米颗粒(AuNP)复合作为生物界面，辣根过氧化物酶(HRP)标记的抗体标记AuNP(HRP-AB2-AuNP)生物偶联物作为一种新的信号标记。信号标签上的大量HRP可以催化H_2O_2氧化对苯二酚(HQ)，从而产生放大的还原电流。催化还原电流与固定在表面的辣根过氧化物酶的量有关，而辣根过氧化物酶本身又与干扰素-γ的浓度有关。在优化的条件下，该免疫传感器具有较高的灵敏度和线性范围，检测下限为0.048.1~10,000 pg/mL。该免疫传感器对临床血清样品的检测结果与参考值符合较好。因此，该免疫传感器在结核病的早期诊断和控制中具有良好的临床应用价值。
Tuberculosis is the most frequent cause of infection-related death worldwide. A new disposable electrochemical immunosensor with low cost and simple fabrication was proposed to detect interferon-γ (IFN-γ). Diallyldimethylammonium chloride (PDDA) and Au nanoparticle (AuNP) composite were used to provide an efficient biointerface, horseradish peroxidase (HRP)-labeled antibody-conjugated AuNP (HRP-Ab2-AuNP) bioconjugates were used as a novel signal tag. The large amounts of HRP on the signal tag can catalyze the oxidation of Hydroquinone (HQ) by H2O2, which can induce an amplified reductive current. The catalytic reduction current was related to the amount of HRP immobilized on the surface, which itself was related to the concentration of IFN-γ. Under optimized conditions, the proposed immunosensor showed a high sensitivity and a linear range of 0.1–10,000 pg/mL with a detection limit of 0.048 pg/mL. The assay results of clinical serum samples obtained by the immunosensor were in acceptable agreement with the reference values. Therefore, the immunosensor possessed excellent clinical value in early diagnosis and control of tuberculosis.