3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients
3D printing of soft lithography mold for rapid production of polydimethylsiloxane-based microfluidic devices for cell stimulation with concentration gradients
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DOI：
10.1007/s10544-015-9928-y
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发表时间：
2015-04-01
影响因子：
2.8
通讯作者：
Chen, Yong
中科院分区：
文献类型：
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作者：
Kamei, Ken-ichiro;Mashimo, Yasumasa;Chen, Yong
三维（3D）打印优于传统技术，用于制造复杂结构，例如用于组织工程和药物筛选中的未来应用的3D微通道。我们的目标是将这项技术应用于使用聚二甲基硅氧烷（PDMS）的基于细胞的测定，聚二甲基硅氧烷是用于制造细胞培养实验所用微通道的最常用材料。PDMS的有用性质包括生物相容性、透气性和透明度。我们开发了一种简单而强大的协议，使用3D打印生产的软光刻模具来生成基于PDMS的设备。然后生成3D化学梯度以刺激限制在微通道中的细胞。我们证明，生长因子的浓度梯度，在体内细胞/组织功能的重要调节剂，影响人胚胎干细胞的存活和生长。因此，这种用于生成3D浓度梯度的方法可能对组织工程和药物筛选具有强烈的影响。
Three-dimensional (3D) printing is advantageous over conventional technologies for the fabrication of sophisticated structures such as 3D micro-channels for future applications in tissue engineering and drug screening. We aimed to apply this technology to cell-based assays using polydimethylsiloxane (PDMS), the most commonly used material for fabrication of micro-channels used for cell culture experiments. Useful properties of PDMS include biocompatibility, gas permeability and transparency. We developed a simple and robust protocol to generate PDMS-based devices using a soft lithography mold produced by 3D printing. 3D chemical gradients were then generated to stimulate cells confined to a micro-channel. We demonstrate that concentration gradients of growth factors, important regulators of cell/tissue functions in vivo, influence the survival and growth of human embryonic stem cells. Thus, this approach for generation of 3D concentration gradients could have strong implications for tissue engineering and drug screening.