Genetic and molecular biological analysis of protein-protein interactions in coronavirus assembly.
Genetic and molecular biological analysis of protein-protein interactions in coronavirus assembly.
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DOI：
10.1007/978-0-387-33012-9_29
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发表时间：
2006
影响因子：
--
通讯作者：
Hsue B
中科院分区：
文献类型：
--
作者：
Masters PS;Kuo L;Ye R;Hurst KR;Koetzner CA;Hsue B
人们已经采取了许多方法来阐明典型结构蛋白S、M、E和N与基因组RNA之间的相互作用网络，这些相互作用导致病毒粒子的组装。最早的努力是对纯化的病毒粒子的成分进行分离和再结合。在这些研究之后，对表达的蛋白质或来自病毒感染细胞的蛋白质进行了分子遗传和免疫共沉淀分析。最近，反向遗传技术已经成为可能。本章将简要回顾目前对CoV组装的理解，重点介绍我们实验室在该领域许多其他小组所做工作的一些最新结果。
A number of approaches have been taken to elucidate the network of interactions, among the canonical structural proteins S, M, E, and N, and the genomic RNA, that lead to assembly of virions. The earliest efforts employed the fractionation and reassociation of components of purified virions. These studies were followed by molecular genetic and co-immunoprecipitation analyses of expressed proteins or proteins from virus-infected cells. More recently, reverse-genetic techniques have become available. This chapter will briefly review the current understanding of CoV assembly, highlighting some recent results from our laboratory in the context of work that has been done by numerous other groups in this field.