Effect of collagen fibril formation on bioresorbability of hydroxyapatite/collagen composites
Effect of collagen fibril formation on bioresorbability of hydroxyapatite/collagen composites
复制标题
DOI：
10.1007/s10856-007-3011-z
复制
发表时间：
2007-08
期刊：
影响因子：
--
通讯作者：
Shunji Yunoki;E. Marukawa;T. Ikoma;S. Sotome;Hongsong Fan;Xing‐dong Zhang;K. Shinomiya;J. Tanaka
中科院分区：
文献类型：
--
作者：
Shunji Yunoki;E. Marukawa;T. Ikoma;S. Sotome;Hongsong Fan;Xing‐dong Zhang;K. Shinomiya;J. Tanaka
多孔羟基磷灰石/胶原(HAP/Col)复合材料是一种很有前途的生物材料和骨组织工程支架材料。研究了Col纤维在多孔复合材料中的形成对生物再吸收性能和机械强度的影响。在中性条件下，将自组织HAP/Col纳米复合材料与磷酸二氢钠缓冲液在37℃孵育时形成原纤维，导致混合物凝胶化。采用冷冻干燥技术制备了有孵化和未孵化的多孔复合材料，并形成了宏观开孔。经脱水热处理后的多孔复合材料的抗压强度(34.1±11.6 kPa)明显高于未孵化时的抗压强度(28.0±33.3 kpa)，这主要是由于胶原纤维的形成所致。体内的生物吸收能力与胶原酶的体外实验结果基本一致。
Porous hydroxyapatite/collagen (HAp/Col) composite is a promising biomaterial and a scaffold for bone tissue engineering. The effect of fibril formation of Col in the porous composite on bioresorbability and mechanical strength was investigated. The fibril formation, in mixing a self-organized HAp/Col nanocomposite and sodium phosphate buffer at a neutral condition, occurred during incubation at 37 °C, resulting in gelation of the mixture. The porous composites with and without the incubation were obtained by freeze-drying technique, in which macroscopic open pores were formed. The compressive strength of the porous composite with the incubation (34.1 ± 1.6 kPa) was significantly higher than that without the incubation (28.0 ± 3.3 kPa) due to the fibril formation of Col. The implantations of the porous composites treated with a dehydrothermal treatment in bone holes revealed that bioresorption was clearly depended on the fibril formation. The bioresorbability in vivo was almost matched to the in vitro test using enzymatic reaction of collagenase.