Expression genome-wide association study identifies that phosphatidylinositol-derived signalling regulates ALUMINIUM SENSITIVE3 expression under aluminium stress in the shoots of Arabidopsis thaliana
Expression genome-wide association study identifies that phosphatidylinositol-derived signalling regulates ALUMINIUM SENSITIVE3 expression under aluminium stress in the shoots of Arabidopsis thaliana
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DOI：
10.1016/j.plantsci.2020.110711
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发表时间：
2021-01-01
期刊：
影响因子：
5.2
通讯作者：
Kobayashi, Yuriko
中科院分区：
文献类型：
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作者：
Sadhukhan, Ayan;Agrahari, Raj Kishan;Kobayashi, Yuriko
为了鉴定导致铝（Al）诱导耐铝基因ALS 3的未知调控机制，我们在Al存在下对95个拟南芥种质的芽中的ALS 3进行了表达全基因组关联研究（eGWAS）。eGWAS使用混合线性模型进行，采用反向遗传学方法验证了940个全基因组单核苷酸多态性（SNP）及其关联结果。我们发现许多来自eGWAS的SNPs与磷脂酰肌醇代谢以及应激信号转导相关的基因相关，包括在共表达网络中相互连接的Ca 2+信号。其中，PLC 9、CDPK 32、ANAC 071、DIR 1和假设蛋白（AT 4G 10470）具有与ALS 3表达水平变异显著相关的氨基酸序列/基因表达水平多态性。此外，T-DNA插入突变体的PLC 9，CDPK 32，和ANAC 071抑制地上部ALS 3的表达在铝的存在下。这项研究阐明了ALS 3的表达在地上部的调控机制，并提供了在铝胁迫下参与磷脂酰肌醇衍生的信号转导的遗传证据。
To identify unknown regulatory mechanisms leading to aluminium (Al)-induction of the Al tolerance gene ALS3, we conducted an expression genome-wide association study (eGWAS) for ALS3 in the shoots of 95 Arabidopsis thaliana accessions in the presence of Al. The eGWAS was conducted using a mixed linear model with 145,940 genome-wide single nucleotide polymorphisms (SNPs) and the association results were validated using reverse genetics. We found that many SNPs from the eGWAS were associated with genes related to phosphatidylinositol metabolism as well as stress signal transduction, including Ca2+ signals, inter-connected in a co-expression network. Of these, PLC9, CDPK32, ANAC071, DIR1, and a hypothetical protein (AT4G10470) possessed amino acid sequence/ gene expression level polymorphisms that were significantly associated with ALS3 expression level variation. Furthermore, T-DNA insertion mutants of PLC9, CDPK32, and ANAC071 suppressed shoot ALS3 expression in the presence of Al. This study clarified the regulatory mechanisms of ALS3 expression in the shoot and provided genetic evidence of the involvement of phosphatidylinositol-derived signal transduction under Al stress.