ANPs effect on MARCKS and StAR phosphorylation in agonist-stimulated glomerulosa cells.
ANPs effect on MARCKS and StAR phosphorylation in agonist-stimulated glomerulosa cells.
复制标题
ANPs 对激动剂刺激的肾小球细胞中 MARCKS 和 StAR 磷酸化的影响。
DOI：
10.1016/s0303-7207(01)00454-3
复制
发表时间：
2001
影响因子：
4.1
通讯作者：
Kent,P
中科院分区：
文献类型：
--
作者：
Calle,RA;Bollag,WB;White,S;Betancourt-Calle,S;Kent,P
心房利钠肽（ANP）是一种抑制所有生理激动剂诱导的醛固酮分泌的心脏激素。本研究的目的是探讨anp在AngII或K+刺激的肾小球细胞中诱导的肉豆浆酰化富丙氨酸c激酶底物（MARCKS）和类固醇急性调节蛋白（StAR）磷酸化的变化。结果表明，在K+刺激的细胞中，ANP完全抑制MARCKS的磷酸化，部分抑制StAR的磷酸化。ANP也部分抑制MARCKS磷酸化，但不影响受AngII刺激的细胞的StAR磷酸化。这些作用似乎与cgmp无关，至少部分依赖于蛋白激酶C （PKC）的抑制。据我们所知，这是ANP在[32P]标记细胞中调节MARCKS或StAR磷酸化的首次报道。这些数据也支持ANP抑制醛固酮分泌的假设，醛固酮是胆固醇转运到线粒体的一个步骤。
Atrial natriuretic peptide (ANP) is a cardiac hormone that inhibits aldosterone secretion induced by all physiologic agonists. The purpose of this study is to explore ANP-induced changes in the phosphorylation of myristoylated alanine-rich C-kinase substrate (MARCKS) and the steroidogenic acute regulatory protein (StAR), in AngII or K+-stimulated glomerulosa cells. The data show that ANP completely inhibits the phosphorylation of MARCKS and partially inhibits that of StAR in cells stimulated with K+. ANP also partially inhibits MARCKS phosphorylation but does not affect StAR phosphorylation in cells stimulated with AngII. These effects appear to be cGMP-independent and at least partially dependent on inhibition of protein kinase C (PKC). To our knowledge, this is the first report of ANP modulating either MARCKS or StAR phosphorylation in [32P]-labeled cells. The data also support the hypothesis that ANP inhibits aldosterone secretion acting as a step involved in cholesterol transport to the mitochondria.