Design and synthesis of environmentally sensitive fluorescent 2-naphthylethynylated 2'-deoxyadenosine: Detection of target DNA via changes in fluorescence intensity and wavelength
Design and synthesis of environmentally sensitive fluorescent 2-naphthylethynylated 2'-deoxyadenosine: Detection of target DNA via changes in fluorescence intensity and wavelength
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环境敏感荧光2-萘乙炔基化2-脱氧腺苷的设计与合成：通过荧光强度和波长的变化检测目标DNA
DOI：
10.1016/j.bmcl.2015.11.037
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发表时间：
2016
影响因子：
2.7
通讯作者：
Yoshio Saito
中科院分区：
文献类型：
--
作者：
Azusa Suzuki;Ryota Kozakai;Tatsuya Aso;Isao Saito;Takuya Takeda;Yoshio Saito
合成了多种C2-萘乙基化的2‘-脱氧腺苷作为环境敏感的荧光核苷，并对它们的光物理性质进行了研究。在合成的ESF核苷中，有4个具有很强的溶致变色性，其中两个被引入到寡核苷酸(ODN)中。这些ODN探针能够通过荧光强度和波长的明显变化来检测目标DNA，并作为有效的报告探针。
Various C2-naphthylethynylated 2′-deoxyadenosines were synthesized as environmentally sensitive fluorescent (ESF) nucleosides and their photophysical properties were examined. Among the ESF nucleosides synthesized, four exhibited strong solvatochromicity, two of which were incorporated into oligodeoxynucleotides (ODNs). These ODN probes were able to detect target DNA through distinct changes in fluorescence intensity and wavelength and acted as effective reporter probes.