Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.
Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.
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DOI：
10.1007/978-1-4939-3606-9_4
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发表时间：
2016
影响因子：
--
通讯作者：
N. Onai;T. Ohteki
中科院分区：
文献类型：
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作者：
N. Onai;T. Ohteki
树突状细胞（DC）在稳态淋巴器官中包括两个主要亚群，常规DC（cDC）和浆细胞样DC（pDC）。这些细胞具有短的半衰期，因此需要从造血干细胞和祖细胞连续产生。最近，我们在小鼠骨髓中发现了DC限制性祖细胞，称为共同DC祖细胞（CDPs）。CDP可以基于造血细胞因子受体从小鼠骨髓中分离，所述造血细胞因子受体例如Flt 3（Fms相关酪氨酸激酶3）（CD 135）、c-kit（CD 117）、M-CSF（巨噬细胞集落刺激因子）受体（CD 115）和IL-7（白细胞介素-7）受体-α（CD 127）。CDPs包括两种祖细胞，CD 115 +CDPs和CD 115 −CDPs，并且在体外和体内仅产生DC亚群。前者CDPs是cDC的主要来源，而后者CDPs是体内pDC的主要来源。在此，我们提供了一种从小鼠中分离树突状细胞祖细胞和骨髓祖细胞的方法。
Dendritic cells (DCs) comprise two major subsets, conventional DC (cDC) and plasmacytoid DC (pDC) in the steady-state lymphoid organ. These cells have a short half-life and therefore, require continuous generation from hematopoietic stem cells and progenitor cells. Recently, we identified DC-restricted progenitors called common DC progenitors (CDPs) in the bone marrow of mouse. The CDPs can be isolated from mouse bone marrow based on the hematopoietic cytokine receptors, such as Flt3 (Fms-related tyrosine kinase 3) (CD135), c-kit (CD117), M-CSF (macrophage colony-stimulating factor) receptor (CD115), and IL-7 (interleukin-7) receptor-α (CD127). The CDPs comprise of two progenitors, CD115+CDPs and CD115−CDPs, and give rise to only DC subsets in both in vitro and in vivo. The former CDPs are the main source of cDC, while the later CDPs are the main source of pDC in vivo. Here, we provide a protocol for the isolation of dendritic cell progenitor and bone marrow progenitor cells from mouse.