Phototoxicity and photoinactivation of blebbistatin in UV and visible light
Phototoxicity and photoinactivation of blebbistatin in UV and visible light
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DOI：
10.1016/j.bbrc.2004.06.045
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发表时间：
2004-07-30
影响因子：
3.1
通讯作者：
Kolega, J
中科院分区：
文献类型：
--
作者：
Kolega, J
Blebbistatin是最近发现的一种选择性的肌球蛋白II抑制剂。由于Blebbistatin很可能在细胞骨架动力学研究中广泛应用于荧光成像，因此对其与常见激发波长的兼容性进行了检测。用365和450-490 nm照射牛主动脉内皮细胞，但不照射510-560或590-650 nm，可引起细胞死亡，且呈剂量依赖性。在365 nm和450-490 nm的光照射下，灯盏花素的吸收光谱和发射光谱均发生改变，但生成的化合物无毒。此外，光反应的灯泡抑素不再干扰细胞内肌球蛋白的分布，表明失去了药理活性。荧光显微镜显示，在光照下，Blebbistatin结合到细胞和蛋白涂层玻璃上，这表明毒性可能是由于Blebbistatin与细胞蛋白的光诱导反应引起的。只有在仔细考虑这些光化学效应的情况下才能使用灯盏花素。(C)2004 Elsevier Inc.保留所有权利。
Blebbistatin was recently identified as a selective, cell-permeant inhibitor of myosin II. Because blebbistatin is likely to be used extensively with fluorescence imaging in studies of cytoskeletal dynamics, its compatibility with common excitation wavelengths was examined. Illumination of blebbistatin-treated bovine aortic endothelial cells at 365 and 450-490 nm, but not 510-560 or 590-650 nm, caused dose-dependent cell death. Illumination of blebbistatin alone at 365 and 450-490 nm changed its absorption and emission spectra, but the resultant compounds were not toxic. In addition, photoreacted blebbistatin no longer disrupted myosin distribution in cells, indicating loss of pharmacological activity. Fluorescence microscopy showed that upon illumination, blebbistatin became bound to cells and to protein-coated glass, suggesting that toxicity may arise from light-induced reaction of blebbistatin with cell proteins. Blebbistatin should be used only with careful consideration of these photochemical effects. (C) 2004 Elsevier Inc. All rights reserved.