IN-VIVO RESTITUTION OF AIRWAY EPITHELIUM
IN-VIVO RESTITUTION OF AIRWAY EPITHELIUM
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DOI：
10.1007/s004410050427
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发表时间：
1995-08-01
影响因子：
3.6
通讯作者：
PERSSON, CGA
中科院分区：
文献类型：
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作者：
ERJEFALT, JS;ERJEFALT, I;PERSSON, CGA
上皮脱落发生在健康和广泛的炎症性气道疾病中。本研究描述了豚鼠气管脱落样上皮剥落后的深度上皮化、再上皮化和相关事件。使用口气管钢探针对800 μ m宽的气管区域进行机械深度切除，没有出血或损伤基膜。用扫描电镜、透射电镜和光镜观察再上皮化。免疫染色检测神经纤维。用[H-3]胸腺嘧啶放射自显影法分析细胞增殖情况。在去除上皮细胞后，伤口边缘的分泌上皮细胞和纤毛上皮细胞（可能是基底上皮细胞）立即去分化，变平并在剥落的基底膜上迅速迁移（2-3 μ m/min）。在8-15小时内，一个新的扁平上皮覆盖了整个深上皮区。30 h时，建立了紧密的上皮屏障，5天后，上皮完全再分化。完全迁移后，恢复区以下的上皮细胞和成纤维细胞/平滑肌有丝分裂活性增加。上皮内含有降钙素基因相关肽的神经纤维在30小时内出现神经再生。我们得出结论：(1)可在体内产生可重复的脱落样剥落，没有出血或基底膜损伤；(2)分泌细胞和纤毛细胞通过去分化和迁移参与再上皮化；(3)体内初始迁移速度非常快；(4)脱落样剥落可能引起强烈的分泌和渗出反应，以及上皮细胞和成纤维细胞/平滑肌的增殖。气道上皮的快速恢复可能依赖于微循环和神经支配的作用。
Epithelial shedding occurs in health and, extensively, in inflammatory airway diseases. This study describes deepithelialisation, reepithelialisation and associated events in guinea-pig trachea after shedding-like epithelial denudation in vivo. Mechanical deepithelialisation of an 800-mu m wide tracheal zone was carried out using an orotracheal steel probe without bleeding or damage to the basement membrane. Reepithelialisation was studied by scanning- and transmission electron microscopy and light microscopy. Nerve fibres were examined by immunostaining. Cell proliferation was analysed by [H-3]-thymidine autoradiography. Immediately after epithelial removal secretory and ciliated (and presumably basal) epithelial cells at the wound margin dedifferentiated, flattened and migrated rapidly (2-3 mu m/min) over the denuded basement membrane. Within 8-15 h a new, flattened epithelium covered the entire deepithelialised zone. At 30 h a tight epithelial barrier was established and after 5 days the epithelium was fully redifferentiated. After completed migration an increased mitotic activity occurred in the epithelium and in fibroblasts/smooth muscle beneath the restitution zone. Reinnervating intraepithelial calcitonin gene-related peptide-containing nerve fibres appeared within 30 h. We conclude that (1) reproducible shedding-like denudation, without bleeding or damage to the basement membrane, can be produced in vivo; (2) secretory and ciliated cells participate in reepithelialisation by dedifferentiation and migration; (3) the initial migration is very fast in vivo; (4) shedding-like denudation may cause strong secretory and exudative responses as well as proliferation of epithelium, and fibroblasts/smooth muscle. Rapid restitution of airway epithelium may depend on contributions from the microcirculation and innervation.