Measurement oftrans and other isomeric unsaturated fatty acids in butterand margarine
Measurement oftrans and other isomeric unsaturated fatty acids in butterand margarine
复制标题
黄油和人造黄油中反式和其他异构不饱和脂肪酸的测量
DOI：
--
复制
发表时间：
1978
期刊：
影响因子：
--
通讯作者：
T. Dairiki
中科院分区：
文献类型：
--
作者：
L. Smith;W. Dunkley;A. Franke;T. Dairiki
本文介绍了不饱和脂肪酸的顺式和反式异构体的气-液色谱分析方法，这些不饱和脂肪酸在饱和脂肪酸甲酯、单烯脂肪酸甲酯、二烯脂肪酸甲酯和多烯脂肪酸甲酯分馏后，用银薄层层析进行测定。为了检验其可靠性，该方法被用来定量测量已知的含有饱和脂肪酸的简单甘油三酯混合物中不饱和脂肪酸的过渡异构体14：1、16：1、18：1和18：2。给出了五个人造黄油和五个黄油样品的分析结果，以及反式脂肪酸浓度的红外光谱分析、共轭脂肪酸浓度的紫外分光光度分析和强制顺-亚甲基间断脂肪酸浓度的酶分析结果。银化薄层-气相色谱联用方法适用于乳脂等复杂食品油脂中主要脂肪酸的测定。
A procedure is described for gas liquid chromatographic determination of cis andtrans isomers of unsaturated fatty acids after fractionation of the saturated, monenoic, dienoic, and polyenoic fatty acid methyl esters by argentation thin layer chromatography. To test its reliability, the procedure was used for quantitative measurement of transisomers of unsaturated fatty acids in a known mixture of simple triglycerides containing saturated fatty acids from 4:0 to 24:0 andcis andtrans isomers of 14:1. 16:1, 18:1, and 18:2. Results of the analyses of five margarine and five butter samples are presented, together with results of infrared spectrophotometric analyses fortrans fatty acid concentrations, ultraviolet spectrophotometric analyses for conjugated fatty acid concentrations, and enzymatic analyses forcis-cis-methylene interrupted fatty acid concentrations. The combined argentation thin layer and gas Chromatographic procedure is suitable for determination of the principal fatty acids in complex food lipids such as milk fat.