Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish
Vesicular monoamine transporter 2 (Vmat2) knockdown elicits anxiety-like behavior in zebrafish
复制标题
囊泡单胺转运蛋白 2 (Vmat2) 敲低会引发斑马鱼的焦虑样行为
DOI：
10.1016/j.bbrc.2016.01.079
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发表时间：
2016
影响因子：
3.1
通讯作者：
Liu Chun-Feng
中科院分区：
文献类型：
--
作者：
Wang Yali;Li Siyue;Liu Wenwen;Wang Fen;Hu Li-Fang;Zhong Zhao-min;Wang Han;Liu Chun-Feng
囊泡单胺转运蛋白2(VMAT2)广泛分布于中枢神经系统，负责将递质摄取到囊泡中。然而，Vmat2缺乏是否与焦虑有关的研究很少，特别是在斑马鱼中。在这里，我们报告了Vmat2杂合突变斑马鱼表现出焦虑样的行为。在新的坦克测试中，突变体在顶部区域花费的时间更少，到达顶部的潜伏期更长。在明/暗盒测试中，它们一致地表现出暗回避，在亮区的持续时间更长，在两个区之间的杂交次数增加。单胺浓度分析表明，VMAT突变体的多巴胺(DA)、5-羟色胺(5-HT)和去甲肾上腺素(NE)等单胺类神经递质及其代谢物水平降低。综上所述，这些发现提示Vmat2杂合突变斑马鱼可能是一种新的焦虑模型，这可能与低水平的DA、5-HT和NE有关。
Vesicular monoamine transporter 2 (Vmat2) is widely distributed in the central nervous system, and responsible for uptaking transmitters into the vesicles. However, whetherVmat2-deficiency is related to the anxiety is rarely investigated, especially in zebrafish. Here, we reportedVmat2heterzygous mutant zebrafish displayed anxiety-like behavior. The mutants spent less time in the top area and took longer latency to the top in the novel tank test. Consistently, they showed dark avoidance in the light/dark box test, with longer duration in the light zone and increased number of crossing between the two zones. Monoamine concentration analysis showed that the levels of monoamine neurotransmitters including dopamine (DA), 5-hydroxy tryptamine (5-HT) and norepinephrine (NE), as well as their metabolites were decreased in VMAT mutants. Taken together, these findings suggest thatVmat2heterzygous mutant zebrafish may serve as a new model of anxiety, which may be related with the low level of DA, 5-HT and NE.