High-performance thin layer chromatographic method for quantitative determination of curcuminoids in Curcuma longa germplasm
High-performance thin layer chromatographic method for quantitative determination of curcuminoids in Curcuma longa germplasm
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DOI：
10.1016/j.foodchem.2008.07.051
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发表时间：
2009-03-15
期刊：
影响因子：
8.8
通讯作者：
Bandyopadhyay, A.
中科院分区：
文献类型：
--
作者：
Paramasivam, M.;Poi, R.;Bandyopadhyay, A.
对姜黄根茎的植物化学研究导致分离出具有药理活性的姜黄素，即姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。这些是通过索氏提取、柱色谱、结晶从姜黄根茎中分离出来的，并通过光谱研究进行鉴定。采用HPTLC方法分析姜黄素的纯度。该方法采用预涂硅胶60GF(254)作为固定相的TLC铝板。溶剂系统由氯仿：甲醇（48：2，v/v）组成。发现该系统给出了姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素的致密斑点（R-F 值分别为 0.66 +/- 0.02、0.48 +/- 0.02 和 0.30 +/- 0.02）。类姜黄素的光密度分析在 425 nm 处以吸收-反射检测模式进行。对七种不同的姜黄种质进行了分析，以检测这三种姜黄素的百分比。 (C) 2008 Elsevier Ltd. 保留所有权利。
Phytochemical investigation of the rhizomes of Curcuma longa leads to the isolation of pharmacologically active curcuminoids viz Curcumin, demethoxycurcumin and bisdemethoxycurcumin. These were isolated from turmeric rhizomes by soxhlet extraction followed by column chromatography, crystallisation and identified by spectroscopic studies. The purity of the curcuminoids was analysed by HPTLC method. The method employed TLC aluminium plates precoated with silica gel 60GF(254) as the stationary phase. The solvent system consisted of chloroform: methanol (48:2, v/v). This system was found to give compact spots for curcumin, demethoxycurcumin and bisdemethoxycurcumin (R-F value of 0.66 +/- 0.02, 0.48 +/- 0.02 and 0.30 +/- 0.02), respectively. Densitometric analysis of curcuminoids was carried out in the absorption-reflection detection mode at 425 nm. Seven different germplasm of turmeric were analysed to detect the percentage of these three curcuminoids. (C) 2008 Elsevier Ltd. All rights reserved.