Characterization of virus-induced interferon-gamma responses in mice previously infected with murine cytomegalovirus.
Characterization of virus-induced interferon-gamma responses in mice previously infected with murine cytomegalovirus.
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先前感染鼠巨细胞病毒的小鼠中病毒诱导的干扰素-γ反应的表征。
DOI：
10.1086/318830
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Biegel,D
中科院分区：
文献类型：
--
作者：
Shanley,JD;Shanley,JA;Albert,G;Biegel,D
这些研究表明，在体外刺激小鼠巨细胞病毒（MCMV）免疫小鼠的脾细胞与MCMV感染的成纤维细胞诱导产生干扰素（IFN）-γ。这种反应对MCMV是特异性的，不普遍适用于异源病毒，也不受H-2限制。早期和晚期CMV抗原均诱导IFN-γ。在体外细胞耗竭和直接细胞选择实验中，T淋巴细胞负责IFN-γ的产生。尽管CD 4和CD 8细胞似乎都是诱导反应所必需的，但释放IFN-γ的细胞亚群尚未确定。在体内，这种IFN-γ应答在急性感染后早期出现，并持续约1年。在T细胞缺陷小鼠中未观察到该反应。因此，先前的MCMV感染导致暴露于MCMV抗原的脾细胞中的病毒特异性IFN-γ应答。这些观察结果的病理生理意义正在研究中
These studies demonstrate that in vitro stimulation of spleen cells from murine cytomega-lovirus (MCMV) immune mice with MCMV-infected fibroblasts induces production of interferon (IFN)–γ. This response is specific to MCMV, is not generalized to heterologous viruses, and also is not H-2 restricted. Both early and late CMV antigens induce IFN-γ. In in vitro cell depletion and direct cell selection experiments, T lymphocytes were responsible for IFN-γ production. Although both CD4 and CD8 cells appear to be required to induce the response, the cell subset that releases the IFN-γ is not yet undefined. In vivo, this IFN-γ response appears early after acute infection and persists ⩾1 year. The response is not seen in T cell–deficient mice. Thus, previous MCMV infection results in a virus-specific IFN-γ response in spleen cells exposed to MCMV antigens. The pathophysiologic significance of these observations is now under study